The association between single nucleotide polymorphism in vitamin D receptor and calcium oxalate urolithiasis in dogs

• Published in: Journal of veterinary internal medicine Vol. 35; no. 5; pp. 2263 - 2270
• Main Authors: Chamsuwan, Sumonwan, Angkanaporn, Kris, Dissayabutra, Thasinas, Chuaypen, Natthaya, Buranakarl, Chollada
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.09.2021; Wiley
• Subjects: Automation; blood serum; calcium oxalate; case-control studies; confidence interval; Creatinine; Electrolytes; genes; genetic variation; genotype; Genotype & phenotype; Homeostasis; Kidney stones; metabolism; Nutrition research; odds ratio; Plasma; Polymorphism; quantitative polymerase chain reaction; renal calculi; risk; single nucleotide polymorphism; SMALL ANIMAL; Software; stone; Thermal cycling; Ultrasonic imaging; Urine; Urogenital system; Variables; VDR; veterinary medicine; Veterinary services; Vitamin D; United States > US; Massachusetts
• ISSN: 0891-6640; 1939-1676; 1939-1676
• DOI: 10.1111/jvim.16225

Background Polymorphisms of the vitamin D receptor (VDR) are associated with calcium oxalate (CaOx) nephrolithiasis in humans. Objectives To investigate the association between VDR polymorphisms and susceptibility to CaOx urolithiasis in dogs. Animals Thirty‐five dogs with CaOx urolithiasis were compared with 40 stone‐free dogs. Methods This was a case‐control study. Two VDR gene polymorphisms (rs851998024 and rs852900542) were detected by specific TaqMan real‐time polymerase chain reaction assay, and their relationship with serum 1,25‐dihydroxyvitamin D, serum and urinary electrolyte concentrations was evaluated. Results The distribution of the rs852900542 polymorphism was significantly different between the case and the control dogs (x2 = 6.369, P = .04). Dogs with a CC or CT genotype had an increased risk of CaOx stones than those with the TT genotype (odds ratio = 3.82, 95% confidence interval 1.04‐13.98). The CaOx dogs with the TT genotype had a significantly lower urinary calcium‐to‐creatinine ratio than the CT+CC genotypes. 1,25‐(OH)2D concentrations did not differ between the cases and the controls (308.7 ± 217.4 vs 286.7 ± 185.1 pg/mL, P = .45). Conclusions and Clinical Importance This finding suggests that vitamin D metabolism might play a role in CaOx stone formation in dogs.