Wangia profunda gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from southern Okinawa Trough deep-sea sediment

• Published in: FEMS microbiology letters Vol. 271; no. 1; pp. 53 - 58
• Main Authors: Qin, Qi-Long, Zhao, Dian-Li, Wang, Jing, Chen, Xiu-Lan, Dang, Hong-Yue, Li, Tie-Gang, Zhang, Yu-Zhong, Gao, Pei-Ji
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.06.2007; Blackwell Publishing Ltd; Blackwell; Oxford University Press
• Subjects: a novel genus; Aerobiosis; Bacteria; Bacteriology; Biological and medical sciences; Catalase; Catalase - biosynthesis; China; Deep sea; Deep sea environments; deep-sea sediment; Deoxyribonucleic acid; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Fatty acids; Fatty Acids - analysis; Flavobacteriaceae; Flavobacteriaceae - chemistry; Flavobacteriaceae - classification; Flavobacteriaceae - isolation & purification; Flavobacteriaceae - physiology; Fundamental and applied biological sciences. Psychology; Gene sequencing; Genes, rRNA - genetics; Geologic Sediments - microbiology; Microbiology; Microscopy, Electron, Scanning; Molecular Sequence Data; New species; Oxidoreductases - biosynthesis; Phenotype; Phylogenetics; Phylogeny; Pigments; Pigments, Biological - biosynthesis; Quinones; Quinones - analysis; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; rRNA 16S; Seawater - microbiology; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; southern Okinawa Trough; Systematics; China; Japan; Okinawa; a novel genus; southern Okinawa Trough; deep-sea sediment; Marine environment; Polymerase chain reaction; Insulation; Phenotype; Flavobacteriaceae; DNA; Bacteria; Phylogeny; Sediments
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.2007.00694.x

An orange-pigmented, Gram-negative, nonmotile, strictly aerobic and oxidase- and catalase-positive bacterium (SM-A87T) was isolated from the deep-sea sediment of the southern Okinawa Trough area. The main fatty acids were i15 : 0, i17 : 0 3OH, i15 : 1 G, i17 : 1ω9c, 15 : 0, i15 : 0 3OH and summed feature 3 (comprising i-15 : 0 2OH and/or 16 : 1ω7c). MK-6 was the predominant respiratory quinone. DNA G+C content was 35.8 mol%. Flexirubin-type pigments were absent. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain SM-A87T formed a distinct lineage within the family Flavobacteriaceae, with <93% sequence similarity to the nearest strain of genus Salegentibacter. Moreover, strain SM-A87T could be distinguished from the nearest phylogenetic neighbors by a number of chemotaxonomic and phenotypic properties. On the basis of polyphasic analyses, it is proposed that strain SM-A87T be classified in a novel genus and a new species in the family Flavobacteriaceae, designated Wangia profunda gen. nov., sp. nov. The type strain is SM-A87T (CCTCC AB 206139T=DSM 18752).