Platinum Palladium Bimetallic Nanozymes Stabilized with Vancomycin for the Sensitive Colorimetric Determination of L-cysteine

• Published in: Biomolecules (Basel, Switzerland) Vol. 13; no. 8; p. 1254
• Main Authors: Zhao, Han, Liu, Kai, Zhou, Lijie, Zhang, Tingting, Han, Zengsheng, Wang, Longgang, Ji, Xianbing, Cui, Yanshuai, Hu, Jie, Ma, Guanglong
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.08.2023; MDPI
• Subjects: bimetallic; Catalysis; Chemical properties; colorimetric; Colorimetry; Cysteine; detection; Enzymes; L-cysteine; Measurement; Metals; Methods; Nanomaterials; Nanoparticles; nanozymes; Palladium; PD-1 protein; Platinum; Toxicity; Vancomycin; China
• ISSN: 2218-273X; 2218-273X
• DOI: 10.3390/biom13081254

Many diseases in the human body are related to the level of L-cysteine. Therefore, it is crucial to establish an efficient, simple and sensitive platform for L-cysteine detection. In this work, we synthesized platinum palladium bimetallic nanoparticles (Van-Ptm/Pdn NPs) using vancomycin hydrochloride (Van) as a stabilizer, which exhibited high oxidase-like catalytic activity. In addition, the catalytic kinetics of the Van-Pt1/Pd1 NPs followed the typical Michaelis–Menten equation, exhibiting a strong affinity for 3,3′,5,5′-tetramethylbenzidine substrates. More importantly, we developed a simple and effective strategy for the sensitive colorimetric detection of L-cysteine using biocompatible Van-Pt1/Pd1 NPs. The detection limit was low, at 0.07 μM, which was lower than the values for many previously reported enzyme-like detection systems. The colorimetric method of the L-cysteine assay had good selectivity. The established method for the detection of L-cysteine showed promise for biomedical analysis.