Activation-induced cellular accumulation of histamine in immature but not mature murine mast cells

• Published in: Cellular and molecular life sciences : CMLS Vol. 65; no. 10; pp. 1585 - 1595
• Main Authors: Fitz, L. J, Brennan, A, Wood, C. R, Goldman, S. J, Kasaian, M. T
• Format: Journal Article
• Language: English
• Published: Basel Basel : SP Birkhäuser Verlag Basel 01.05.2008; SP Birkhäuser Verlag Basel; Springer Nature B.V
• Subjects: Animals; Biochemistry; Biomedical and Life Sciences; Biomedicine; Bone Marrow Cells - cytology; Bone Marrow Cells - physiology; Cell Biology; Cell Count; Cell Differentiation - immunology; Cell Proliferation; Cellular biology; Cytoplasmic Granules - metabolism; Enzyme Induction; Enzymes; FcεRI; Female; granule; Histamine; Histamine - metabolism; Histidine decarboxylase; Histidine Decarboxylase - biosynthesis; Histidine Decarboxylase - genetics; Life Sciences; mast cell; Mast Cells - cytology; Mast Cells - immunology; Mast Cells - metabolism; Mast Cells - physiology; Mice; Mice, Inbred C57BL; Peritoneal Cavity - cytology; Research Article; Rodents; mast cell; granule; FcεRI; histamine; Histidine decarboxylase
• ISSN: 1420-682X; 1420-9071
• DOI: 10.1007/s00018-008-8106-2

Mast cell activation involves the rapid release of inflammatory mediators, including histamine, from intracellular granules. The cells are capable of regranulation and multiple rounds of activation. The goal of this study was to determine if there are changes in the content of pre-formed mast cell mediators after a round of activation. After 24 h, the histamine content of bone marrow-derived mast cells (BMMC), but not that of peritoneal mast cells, exceeded the amount in resting cells. Accumulation of histamine in BMMC peaked at 72 h of activation, and returned toward preactivation levels by 96 h. The increase in histamine content was accompanied by an increase in the gene expression of histidine decarboxylase. No increases in β hexosaminidase or murine mast cell protease-6 were observed. These findings indicate that BMMC respond to activation by increasing total cell-associated histamine content. This increase may be important to the response of these cells upon subsequent exposure to antigens.