Methods to capture proteomic and metabolomic signatures from cerebrospinal fluid and serum of healthy individuals

• Published in: Scientific reports Vol. 12; no. 1; p. 13339
• Main Authors: Lilley, Laura M, Sanche, Steven, Moore, Shepard C, Salemi, Michelle R, Vu, Dung, Iyer, Srinivas, Hengartner, Nicolas W, Mukundan, Harshini
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 03.08.2022; Nature Publishing Group UK; Nature Portfolio
• Subjects: BASIC BIOLOGICAL SCIENCES; Biomarkers; Cerebrospinal Fluid; Cohort Studies; Diagnostic tests; Disease; Female; Humans; Injuries; Male; Metabolism; Metabolites; Metabolomics; Nervous System Diseases; Neurological diseases; Neurons; Phlebotomy; Proteins; Proteomics; Traumatic brain injury
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-16598-1

Discovery of reliable signatures for the empirical diagnosis of neurological diseases-both infectious and non-infectious-remains unrealized. One of the primary challenges encountered in such studies is the lack of a comprehensive database representative of a signature background that exists in healthy individuals, and against which an aberrant event can be assessed. For neurological insults and injuries, it is important to understand the normal profile in the neuronal (cerebrospinal fluid) and systemic fluids (e.g., blood). Here, we present the first comparative multi-omic human database of signatures derived from a population of 30 individuals (15 males, 15 females, 23-74 years) of serum and cerebrospinal fluid. In addition to empirical signatures, we also assigned common pathways between serum and CSF. Together, our findings provide a cohort against which aberrant signature profiles in individuals with neurological injuries/disease can be assessed-providing a pathway for comprehensive diagnostics and therapeutics discovery.