Effects of Xylanase Supplementation to Wheat-based Diets on Growth Performance, Nutrient Digestibility and Gut Microbes in Weanling Pigs

• Published in: Animal bioscience Vol. 31; no. 9; pp. 1491 - 1499
• Main Authors: Dong, Bing, Liu, Shaoshuai, Wang, Chunlin, Cao, Yunhe
• Format: Journal Article
• Language: English
• Published: Korea (South) Asian - Australasian Association of Animal Production Societies 01.09.2018; Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST); Asian-Australasian Association of Animal Production Societies; 아세아·태평양축산학회
• Subjects: 16s rRNA Sequencing; Analysis; Bacterial Community Weanling Pigs; Feed research; Feed supplements; Livestock productivity; Methods; Pig feed; Xylanase; Xylanases; 축산학; 16s rRNA sequencing; Weanling pigs; Xylanase; Bacterial community
• ISSN: 1011-2367; 2765-0189; 1976-5517; 2765-0235
• DOI: 10.5713/ajas.17.0867

This study was designed to investigate the effects of an Aspergillus sulphureus xylanase expressed in Pichia pastoris on the growth performance, nutrient digestibility and gut microbes in weanling pigs. A total of 180 weanling pigs (initial body weights were 8.47 ± 1.40 kg) were assigned randomly to 5 dietary treatments. Each treatment had 6 replicates with 6 pigs per replicate. The experimental diets were wheat based with supplementation of 0, 500, 1000, 2000 and 4000 U xylanase/kg. The experiment lasted 28 days (early phase, d 0-14; late phase, d 15-28). In the early phase, compared to the control, average daily gain (ADG) was higher for pigs fed diets supplemented with xylanase and there was a quadratic response in ADG (P<0.05). In the entire phase, ADG was higher for the pigs fed 1000 or 2000 U/kg xylanase compared to the control (P<0.05). The G:F was higher for pigs fed diets supplemented with 1000 or 2000 U/kg xylanase compared to the control (P<0.05). Increasing the amount of xylanase improved the apparent total tract digestibility (ATTD) of dry matter, crude protein, neutral detergent fiber, calcium, and phosphorus during both periods (p<0.05). Xylanase supplementation (2000 U/kg) decreased the proportion of Lachnospiraceae (by 50%) in Firmicutes, but increased Prevotellaceae (by 175%) in Bacteroidetes and almost diminished Enterobacteriaceae (Escherichia-Shigella) in Proteobacteria. Xylanase supplementation increased growth performance and nutrient digestibility up to 2000 U/kg. Supplementation of xylanase (2000 U/kg) decreased the richness of gut bacteria but diminished the growth of harmful pathogenic bacteria, such as Escherichia-Shigella, in the colon.