Modification of an Automated Liquid-Handling System for Reagent-Jet, Nanoliter-Level Dispensing

Reducing the scale of biochemical reactions is becoming commonplace. Examples include the screening of large libraries of chemical compounds or gene sequences. These applications demand the ability to transfer sub-microliter volumes of fluid. To this end, we have modified a Hamilton MICROLAB 2200 wi...

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Published inBioTechniques Vol. 30; no. 4; pp. 878 - 885
Main Authors Hicks, J. Steven, Harker, Brent W, Beattie, Kenneth L, Doktycz, Mitchel J
Format Journal Article
LanguageEnglish
Published Natick, MA Future Science Ltd 01.04.2001
Eaton
Taylor & Francis Group
Subjects
Aluminum Oxide
Biological and medical sciences
Electronics - instrumentation
Fundamental and applied biological sciences. Psychology
Gene Library
General aspects
Instrumentation. Materials. Reagents. Research laboratory organization
Microinjections - instrumentation
Molecular Biology - instrumentation
Oligonucleotide Array Sequence Analysis - instrumentation
Software
Modification
Instrumentation
Automation
Volume
Pipette
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Summary:Reducing the scale of biochemical reactions is becoming commonplace. Examples include the screening of large libraries of chemical compounds or gene sequences. These applications demand the ability to transfer sub-microliter volumes of fluid. To this end, we have modified a Hamilton MICROLAB 2200 with high-speed solenoids and a liquid pressurization system to modulate volume delivery down to the nanoliter level. Additional modifications include the use of sapphire-tipped dispensing nozzles and a high-resolution substage to assist in the construction of DNA microarrays. Techniques for characterizing the dispensed volume are presented.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0736-6205
1940-9818
DOI:10.2144/01304rr05