Laser enhanced photothermal effect of silver nanoparticles synthesized by chemical and green method on Gram-positive and Gram-negative bacteria

• Published in: BMC chemistry Vol. 18; no. 1; pp. 163 - 14
• Main Authors: Mostafa, Elham M., Badr, Y., Ramadan, Marwa A., Hashem, Mohamed M. M., Abo-El-Sooud, Khaled, Deif, Heba N., Faid, Amna H.
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 03.09.2024; BioMed Central Ltd; Springer Nature B.V; BMC
• Subjects: Antibacterial agents; Antifungal agents; Antimicrobial blue laser; Bacteria; Bacterial pneumonia; Chemical activity; Chemical reduction; Chemical synthesis; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Chitosan; Drug resistance in microorganisms; Fourier transforms; Fungicides; Green nanotechnology; Green technology; Infrared spectrometers; Irradiation; Klebsiella; Lasers; Lasers in medicine; Lasers in surgery; Low concentrations; Methods; Multidrug resistance; Nanomedicine; Nanoparticles; Nanotechnology; Photothermal agents; Pneumonia; Semiconductor lasers; Side effects; Silver; Spectrum analysis; Staphylococcal infections; Therapy; Zeta potential; Photothermal therapy; Multidrug resistance; Bacteria; Green nanotechnology; Antimicrobial blue laser; Nanomedicine; Photothermal agents
• ISSN: 2661-801X; 2661-801X
• DOI: 10.1186/s13065-024-01263-7

Purpose The antibacterial properties of silver nanoparticles (AgNPs) are extensively identified. In large quantities, they might be harmful. So many fields of nanotechnology have shown a great deal of interest in the development of an environmentally friendly, efficient method for synthesizing metal nanoparticles. Because of its antibacterial and antifungal properties toward a wide range of microbes, chitosan silver nanoparticles (AgNPs@Cs) constitute a newly developing class of bio-nanostructured hybrid materials. Furthermore, the use of photothermal therapy (PTT) has been suggested as a means of elimination of germs. These light-stimulated treatments are minimally invasive and have a few side effects. In the present work, the antibacterial effect of AgNPs at low concentrations; prepared by chemical and green methods as antimicrobial and photothermal agents in photothermal therapy; with laser irradiation were explored as combined treatment against MRSA, Pseudomonas aeruginosa , and Klebsiella pneumoniae . Methods Silver nanoparticles were produced in two ways. First, by sodium borohydrides, second, by chitosan (as a natural eco-friendly reducing, and capping agent). The nanostructure of AgNPs and AgNPs@Cs was confirmed by UV–visible spectrometer, transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIRs), and direct light scattering (DLS). The antibacterial activity of the prepared nanoparticles and the laser irradiation was tested against three bacterial species of zoonotic importance; MRSA, Pseudomonas aeruginosa , and Klebsiella pneumoniae; and was evaluated by measuring their minimum inhibitory concentrations (MIC). Results Silver nanoparticles produced by the two methods had spherical shapes with nearly the same particle size. The analysis of DLS showed that AgNPs were very stable with zeta potential − 28.8 mv, and 47.7 mv by chemical and chitosan synthesis, respectively. Furthermore, AgNPs@Cs showed higher antibacterial activity toward the tested bacterial species than AgNPs by chemical method. Additionally, the bacterial viability using photothermal laser therapy was reduced compared to laser and AgNPs alone. The bactericidal activities were higher when laser diode was coupled with AgNPs@Cs than by chemical reduction. Conclusion The laser combined treatment had a higher antimicrobial effect than AgNPs alone or laser irradiation alone.