Promotion of cAMP Responsive Element-Binding Protein Activity Ameliorates Radiation-Induced Suppression of Hippocampal Neurogenesis in Adult Mice

• Published in: Toxicological research (Seoul) Vol. 26; no. 3; pp. 177 - 183
• Main Authors: Kim, J.S., Chonnam National University, Gwangju, Republic of Korea, Yang, M.Y., Chonnam National University, Gwangju, Republic of Korea, Cho, J.H., Chonnam National University, Gwangju, Republic of Korea, Kim, S.H., Chonnam National University, Gwangju, Republic of Korea, Kim, J.C., Chonnam National University, Gwangju, Republic of Korea, Shin, T.K., Cheju National University, Jeju, Republic of Korea, Moon, C.J., Chonnam National University, Gwangju, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Singapore 한국독성학회 01.09.2010; Springer Singapore; The Korean Society of Toxicology
• Subjects: Biomedicine; CREB; Hippocampus; IRRADIACION; IRRADIATION; Neurogenesis; Pharmacology/Toxicology; Rolipram; 예방의학; Irradiation; CREB; Rolipram; Neurogenesis; Hippocampus
• ISSN: 1976-8257; 2234-2753
• DOI: 10.5487/TR.2010.26.3.177

This study was performed to examine whether elevated activity of cAMP responsive element-binding protein (CREB) attenuates the detrimental effects of acute gamma (γ)-irradiation on hippocampal neurogenesis and related functions. C57BL/6 male mice were treated with rolipram (1.25 mg/kg, i.p., twice a day for 5 consecutive days) to activate the cAMP/CREB pathway against cranial irradiation (2 Gy), and were euthanized at 24 h post-irradiation. Exposure to γ-rays decreased both CREB phosphorylation and immunohistochemical markers for neurogenesis, including Ki-67 and doublecortin (DCX), in the hippocampal dentate gyrus (DG). However, the rolipram treatment protected from γ-irradiation-induced decreases of CREB phosphorylation, and Ki-67 and DCX immunoreactivity in the hippocampal DG. In an object recognition memory test, mice trained 24 h after acute γ-irradiation (2 Gy) showed significant memory impairment, which was attenuated by rolipram treatment. The results suggest that activation of CREB signaling ameliorates the detrimental effects of acute γ-irradiation on hippocampal neurogenesis and related functions in adult mice.