Isolation and characterization of the adenylate cyclase structural gene of Neurospora crassa

• Published in: Idengaku zasshi Vol. 66; no. 3; pp. 317 - 334
• Main Authors: Koreeda, S. (Tokyo Univ. (Japan). Inst. of Applied Microbiology), Murayama, T, Uno, I
• Format: Journal Article
• Language: English
• Published: Tokyo The Genetics Society of Japan 1991; University of Tokyo, Institute of Applied Microbiology
• Subjects: ADENILATO CICLASA; ADENYLATE CYCLASE; Adenylyl Cyclases - genetics; Adenylyl Cyclases - isolation & purification; Amino Acid Sequence; Base Sequence; Biological and medical sciences; Cloning, Molecular; DNA, Fungal; Escherichia coli - genetics; Fundamental and applied biological sciences. Psychology; GENE; GENES; Genes, Fungal; Genes. Genome; GENETIC ENGINEERING; GENIE GENETIQUE; Genomic Library; INGENIERIA GENETICA; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; NEUROSPORA; Neurospora crassa - enzymology; Neurospora crassa - genetics; Neurospora crassa - growth & development; Polymorphism, Restriction Fragment Length; Restriction Mapping; Sequence Alignment; Nucleotide sequence; Enzyme; Gene product; Homology; Gene organization; Gene expression; Fungi; Adenylate cyclase; Enzymatic activity; Chromosome DNA; Ascomycetes; Neurospora crassa; Molecular cloning; Thallophyta
• ISSN: 0021-504X; 1880-5787
• DOI: 10.1266/jjg.66.317

A single gene (nac) encoding an adenylate cyclase was cloned from the genomic DNA library of Neurospora crassa, using the DNA fragment encoding the catalytic domain of adenylate cyclase of Saccharomyces cerevisiae as a probe. The open reading frame of this gene (6900 base pairs) was interrupted three time by introns. The protein encoded consists of 2300 amino acids and has adenylate cyclase activity. N. crassa adenylate cyclase has a high degree of homology with the catalytic domains of yeast and bovine brain adenylate cyclases.