Plant Virus DNA Replication Processes in Agrobacterium: Insight into the Origins of Geminiviruses?

Agrobacterium tumefaciens, a bacterial plant pathogen, when transformed with plasmid constructs containing greater than unit length DNA of tomato leaf curl geminivirus accumulates viral replicative form DNAs indistinguishable from those produced in infected plants. The accumulation of the viral DNA...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 93; no. 19; pp. 10280 - 10284
Main Authors Rigden, J. E., Dry, I. B., Krake, L. R., Rezaian, M. A.
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences of the United States of America 17.09.1996
National Acad Sciences
National Academy of Sciences
Subjects
Agrobacterium
Bacteria
Biological Evolution
Blotting, Southern
Cloning, Molecular
Deoxyribonucleic acid
DNA
DNA probes
DNA Replication
DNA, Viral - analysis
DNA, Viral - biosynthesis
Evolution
Geminiviridae
Geminiviridae - classification
Geminiviridae - genetics
geminivirus
Genetic mutation
Genome, Viral
Models, Genetic
Open Reading Frames
Plant Viruses - genetics
Plant Viruses - metabolism
Plasmids
Repetitive Sequences, Nucleic Acid
Replication origin
Rhizobium - virology
Tandem repeat sequences
tomato leaf curl virus
Tomatoes
Viral DNA
Viruses
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Summary:Agrobacterium tumefaciens, a bacterial plant pathogen, when transformed with plasmid constructs containing greater than unit length DNA of tomato leaf curl geminivirus accumulates viral replicative form DNAs indistinguishable from those produced in infected plants. The accumulation of the viral DNA species depends on the presence of two origins of replication in the DNA constructs and is drastically reduced by introducing mutations into the viral replication-associated protein (Rep or C1) ORF, indicating that an active viral replication process is occurring in the bacterial cell. The accumulation of these viral DNA species is not affected by mutations or deletions in the other viral open reading frames. The observation that geminivirus DNA replication functions are supported by the bacterial cellular machinery provides evidence for the theory that these circular single-stranded DNA viruses have evolved from prokaryotic episomal replicons.
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ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.93.19.10280