Cystic Fibrosis Transmembrane Conductance Regulator Reduces Microtubule-Dependent Campylobacter jejuni Invasion
is a foodborne pathogen that induces gastroenteritis. Invasion and adhesion are essential in the process of infection leading to gastroenteritis. The mucosal layer plays a key role in the system of defense against efficient invasion and adhesion by bacteria, which is modulated by several ion channel...
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Published in | Infection and immunity Vol. 85; no. 10 |
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Main Authors | , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Society for Microbiology
01.10.2017
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Subjects | |
Online Access | Get full text |
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Summary: | is a foodborne pathogen that induces gastroenteritis. Invasion and adhesion are essential in the process of
infection leading to gastroenteritis. The mucosal layer plays a key role in the system of defense against efficient invasion and adhesion by bacteria, which is modulated by several ion channels and transporters mediated by water flux in the intestine. The cystic fibrosis transmembrane conductance regulator (CFTR) plays the main role in water flux in the intestine, and it is closely associated with bacterial clearance. We previously reported that
infection suppresses CFTR channel activity in intestinal epithelial cells; however, the mechanism and importance of this suppression are unclear. This study sought to elucidate the role of CFTR in
infection. Using HEK293 cells that stably express wild-type and mutated CFTR, we found that CFTR attenuated
invasion and that it was not involved in bacterial adhesion or intracellular survival but was associated with microtubule-dependent intracellular transport. Moreover, we revealed that CFTR attenuated the function of the microtubule motor protein, which caused inhibition of
invasion, but did not affect microtubule stability. Meanwhile, the CFTR mutant G551D-CFTR, which had defects in channel activity, suppressed
invasion, whereas the ΔF508-CFTR mutant, which had defects in maturation, did not suppress
invasion, suggesting that CFTR suppression of
invasion is related to CFTR maturation but not channel activity. When these findings are taken together, it may be seen that mature CFTR inhibits
invasion by regulating microtubule-mediated pathways. We suggest that CFTR plays a critical role in cellular defenses against
invasion and that suppression of CFTR may be an initial step in promoting cell invasion during
infection. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Citation Kido J, Shimohata T, Amano S, Hatayama S, Nguyen AQ, Sato Y, Kanda Y, Tentaku A, Fukushima S, Nakahashi M, Uebanso T, Mawatari K, Takahashi A. 2017. Cystic fibrosis transmembrane conductance regulator reduces microtubule-dependent Campylobacter jejuni invasion. Infect Immun 85:e00311-17. https://doi.org/10.1128/IAI.00311-17. |
ISSN: | 0019-9567 1098-5522 |
DOI: | 10.1128/iai.00311-17 |