Reversibility and safety of KISS1 metastasis suppressor gene vaccine in immunocastration of ram lambs

• Published in: Animal bioscience Vol. 31; no. 6; pp. 835 - 841
• Main Authors: Han, Yan-Guo, Liu, Gui-Qiong, Jiang, Xun-Ping, Xiang, Xing-Long, Huang, Yong-Fu, Nie, Bin, Zhao, Jia-Yu, Nabeel, Ijaz, Tesema, Birhanu
• Format: Journal Article
• Language: English
• Published: Korea (South) Asian - Australasian Association of Animal Production Societies 01.06.2018; Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST); Asian-Australasian Association of Animal Production Societies; 아세아·태평양축산학회
• Subjects: Animal vaccination; Castration; Gene Vaccine; Immunocastration; KISS1 Metastasis Suppressor; Methods; Physiological aspects; Ram Lambs; Rams; Reversibility; Safety; 축산학; Gene Vaccine; Reversibility; Ram Lambs; Safety; Immunocastration; KISS1 Metastasis Suppressor
• ISSN: 1011-2367; 2765-0189; 1976-5517; 2765-0235
• DOI: 10.5713/ajas.17.0629

The aim of this study was to investigate the reversibility and safety of KISS1 metastasis suppressor ( ) gene vaccine in immunocastration. Six eight-week old ram lambs were randomly divided into vaccinated and control groups. The vaccine (1 mg/ram lamb) was injected at weeks 0, 3, and 6 of the study. Blood samples were collected from the jugular vein before primary immunization and at weeks 2, 4, 6, 10, 14, 22, and 30 after primary immunization. All ram lambs were slaughtered at 38 weeks of age, and samples were collected. The specific anti- antibody titers in vaccinated animals were significantly higher and the serum testosterone level was significantly lower than those in the control groups from week 4 to 14 after primary immunization (p<0.05). No significant difference was observed at weeks 22 and 30 after the primary immunization. Similar results were also found for scrotal circumference, testicular weight, length, breadth, and spermatogenesis in seminiferous tubules in week 30 after primary immunization. KS ( -hepatitis B surface antigen ) fusion fragment of gene vaccine was not detected in host cell genomic DNA of 9 tissues of the vaccinated ram lambs by polymerase chain reaction. The effects of gene vaccine in immunocastration were reversible and no integration events were recorded.