Development of a new lactic acid bacterial inoculant for fresh rice straw silage

• Published in: Animal bioscience Vol. 30; no. 7; pp. 950 - 956
• Main Authors: Kim, Jong Geun, Ham, Jun Sang, Li, Yu Wei, Park, Hyung Soo, Huh, Chul-Sung, Park, Byung-Chul
• Format: Journal Article
• Language: English
• Published: Korea (South) Asian - Australasian Association of Animal Production Societies 01.07.2017; Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST); Asian-Australasian Association of Animal Production Societies; 아세아·태평양축산학회
• Subjects: Analysis; Composition; Fermentation; Inoculant; Lactic Acid Bacteria; Lactobacillus plantarum; Methods; Observations; Quality; Rice; Rice Straw Silage; RNA; Silage; 축산학; Fermentation; Inoculant; Lactic Acid Bacteria; Rice Straw Silage; Quality
• ISSN: 1011-2367; 2765-0189; 1976-5517; 2765-0235
• DOI: 10.5713/ajas.17.0287

Effects of newly isolated on the fermentation and chemical composition of fresh rice straw silage was evaluated in this study. Lactic acid bacteria (LAB) from good crop silage were screened by growing them in MRS broth and a minimal medium with low carbohydrate content. Selected LAB (LAB 1821) were Gram-positive, rods, catalase negative, and were identified to be based on their biochemical characteristics and a 16S rRNA analysis. Fresh rice straw was ensiled with two isolated LAB (1821 and 1841), two commercial inoculants (HM/F and P1132) and no additive as a control. After 2 months of storage at ambient temperature, rice straw silages treated with additives were well-preserved, the pH values and butyric and acetic acid contents were lower, and the lactic acid content and lactic/acetic acid ratio were higher than those in the control (p<0.05). Acidity (pH) was lowest, and lactic acid highest, in 1821-treated silage (p<0.05). The NH -N content decreased significantly in inoculant-treated silage (p<0.05) and the NH -N content in 1821-treated silage was lowest among the treatments. The dry matter (DM) content of the control silage was lower than that of fresh rice straw (p<0.05), while that of the 1841- and p1174-inoculant-treated silages was significantly higher than that of HM/F-treated silage. Microbial additives did not have any significant (p>0.05) effect on acid detergent fiber or neutral detergent fiber contents. Crude protein (CP) content and DM digestibility (IVDMD) increased after inoculation of LAB 1821 (p<0.05). LAB 1821 increased the CP, IVDMD, lactic acid content and ratio of lactic acid to acetic acid in rice straw silage and decreased the pH, acetic acid, NH -N, and butyric acid contents. Therefore, adding LAB 1821 improved the fermentation quality and feed value of rice straw silage.