Evaluation of different ionic liquid stationary phases for the analysis of carbohydrates by gas chromatography-mass spectrometry

• Published in: Analytical and bioanalytical chemistry Vol. 411; no. 28; pp. 7461 - 7472
• Main Authors: Rodríguez-Sánchez, Sonia, Soria, Ana C., Lebrón-Aguilar, Rosa, Sanz, Maria L., Ruiz-Matute, Ana I.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.11.2019; Springer; Springer Nature B.V
• Subjects: Analytical Chemistry; Biochemistry; Biological products industry; Carbohydrates; Carbohydrates - analysis; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chromatography; Comparative analysis; Elution; Food Science; Gas chromatography; Gas Chromatography-Mass Spectrometry - methods; Inositols; Instrument industry; Ionic liquids; Ionic Liquids - chemistry; Ions; Laboratory Medicine; Low molecular weights; Mass spectrometry; Mass spectroscopy; Molecular weight; Monitoring/Environmental Analysis; Oligosaccharides; Phases; Plant Extracts - chemistry; Research Paper; Scientific imaging; Selectivity; Spectroscopy; Spectrum analysis; Temperature; United States; Spain; Ionic liquids; Mulberry; Gas chromatography-mass spectrometry; Stationary phases; Hyacinth; Low molecular weight carbohydrates
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-019-02118-0

Five commercial ionic liquid (IL) columns have been evaluated for the first time for the gas chromatography-mass spectrometry (GC-MS) analysis of low molecular weight carbohydrate (LMWC) standards (mono-, di-, and trisaccharides, inositols, and iminosugars). A previous derivatization step was necessary to convert the LMWCs into their volatile and stable derivatives. Compared with conventional GC stationary phases, such as HP-1 and Supelcowax ® 10, IL columns have shown a different selectivity in the separation of target compounds. Among the IL columns, only SLB™-IL82 allowed the elution of all the LMWCs studied. Its performance in terms of peak width and asymmetry, evaluated under different oven temperature conditions, was shown to be dependent on the carbohydrate class considered. As an example of application, a SLB™-IL82 column was successfully used to separate the complex mixtures of LMWCs in hyacinth and mulberry extracts. This column is an interesting alternative to the conventional stationary phases used in the GC analysis of LMWCs in real-world samples. Graphical abstract