Nuclear and nucleolar localization of Saccharomyces cerevisiae ribosomal proteins S22 and S25

• Published in: FEBS letters Vol. 452; no. 3; pp. 335 - 340
• Main Authors: Timmers, Antonius C.J, Stuger, Rogier, Schaap, Peter J, van ’t Riet, Jan, Raué, Hendrik A
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 11.06.1999
• Subjects: Amino Acid Sequence; beta-Galactosidase - analysis; beta-Galactosidase - chemistry; Cell Nucleolus - ultrastructure; Cell Nucleus - ultrastructure; Consensus sequence; Immunohistochemistry; Moleculaire genetica van industriële micro-organismen; Molecular Genetics of Industrial Micro-organisms; Molecular Sequence Data; Nuclear localization sequence; Nucleolus; Peptide Fragments - chemistry; Recombinant Fusion Proteins - analysis; Recombinant Fusion Proteins - chemistry; Ribosomal protein; Ribosomal Proteins - analysis; Ribosomal Proteins - chemistry; Ribosomal Proteins - genetics; Saccharomyces cerevisiae - cytology; Saccharomyces cerevisiae - ultrastructure; Saccharomyces cerevisiae Proteins; VLAG; Yeast; Yeast; Nucleolus; Consensus sequence; Nuclear localization sequence; Ribosomal protein
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(99)00669-9

Nuclear import usually relies on the presence of nuclear localization sequences (NLSs). NLSs are recognized by NLS receptors (importins), which target their substrates to the nuclear pore. We identified the NLSs of the yeast ribosomal proteins S22 and S25 and studied the former by mutational analysis. Furthermore, in S25 the nucleolar targeting information was found to overlap with its NLS. Comparison with previously published data on yeast ribosomal protein NLSs and computer analysis indicates the existence of a novel type of ribosomal protein-specific NLS that differs from the classical Chelsky and bipartite NLSs. The existence of such a ribosomal protein-specific NLS is in accordance with the recent identification of ribosomal protein-specific importins.