Effects of trypsinization and mineralization on intrasynovial tendon allograft healing to bone

• Published in: Journal of orthopaedic research Vol. 33; no. 4; pp. 468 - 474
• Main Authors: Qu, Jin, van Alphen, Nick A., Thoreson, Andrew R., Chen, Qingshan, An, Kai-Nan, Amadio, Peter C., Schmid, Thomas M., Zhao, Chunfeng
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.04.2015
• Subjects: Allografts; Animals; Biomechanical Phenomena; Calcification, Physiologic - physiology; Elasticity; Glycoproteins - analysis; In Vitro Techniques; intrasynovial tendon; Knee Joint - physiopathology; Knee Joint - surgery; mineralization; Rabbits; Random Allocation; Reconstructive Surgical Procedures; tendon-to-bone healing; Tendons - chemistry; Tendons - pathology; Tendons - transplantation; Tibia - physiopathology; Tibia - surgery; trypsinization; Trypsinogen - pharmacology; Wound Healing; trypsinization; mineralization; intrasynovial tendon; tendon-to-bone healing
• ISSN: 0736-0266; 1554-527X
• DOI: 10.1002/jor.22779

ABSTRACT The purpose of the current study was to develop a novel technology to enhance tendon‐to‐bone interface healing by trypsinizing and mineralizing (TM) an intrasynovial tendon allograft in a rabbit bone tunnel model. Eight rabbit flexor digitorum profundus (FDP) tendons were used to optimize the trypsinization process. An additional 24 FDP tendons were stratified into control and TM groups; in each group, 4 tendons were used for in vitro evaluation of TM and 8 were transplanted into proximal tibial bone tunnels in rabbits. The samples were evaluated histologically and with mechanical testing at postoperative week 8. Maximum failure strength and linear stiffness were not significantly different between the control and TM tendons. A thin fibrous band of scar tissue formed at the graft‐to‐bone interface in the control group. However, only the TM group showed obvious new bone formation inside the tendon graft and a visible fibrocartilage layer at the bone tunnel entrance. This study is the first to explore effects of TM on the intrasynovial allograft healing to a bone tunnel. TM showed beneficial effects on chondrogenesis, osteogenesis, and integration of the intrasynovial tendon graft, but mechanical strength was the same as the control tendons in this short‐term in vivo study. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:468–474, 2015.