Plasma profiles in active systemic juvenile idiopathic arthritis: Biomarkers and biological implications

• Published in: Proteomics (Weinheim) Vol. 10; no. 24; pp. 4415 - 4430
• Main Authors: Ling, Xuefeng B, Park, Jane L, Carroll, Tanya, Nguyen, Khoa D, Lau, Kenneth, Macaubas, Claudia, Chen, Edward, Lee, Tzielan, Sandborg, Christy, Milojevic, Diana, Kanegaye, John T, Gao, Susanna, Burns, Jane, Schilling, James, Mellins, Elizabeth D
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH Verlag 01.12.2010; WILEY-VCH Verlag; WILEY‐VCH Verlag; Wiley-VCH
• Subjects: 2-D DIGE; Adolescent; Analytical, structural and metabolic biochemistry; Arthritis, Juvenile - blood; Arthritis, Juvenile - diagnosis; Biological and medical sciences; Biomarkers - blood; Blood Proteins - analysis; Blood Proteins - classification; Child; Child, Preschool; Diagnosis, Differential; Diseases of the osteoarticular system; Fundamental and applied biological sciences. Psychology; Humans; Infant; Inflammatory joint diseases; Kawasaki disease; MALDI-TOF/TOF MS; Medical sciences; Miscellaneous; Proteins; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis; Systemic juvenile idiopathic arthritis; Systems biology; Young Adult; Kawasaki syndrome; Immunopathology; 2-D DIGE; Diseases of the osteoarticular system; Systemic juvenile idiopathic arthritis; Biological marker; Autoimmune disease; Cardiovascular disease; Juvenile rheumatoid arthritis; Inflammatory joint disease; Vascular disease; Chronic; Vasculitis; Kawasaki disease; Systems biology; Systemic disease; Proteomics; MALDI-TOF/TOF MS
• ISSN: 1615-9853; 1615-9861; 1615-9861
• DOI: 10.1002/pmic.201000298

Systemic juvenile idiopathic arthritis (SJIA) is a chronic arthritis of children characterized by a combination of arthritis and systemic inflammation. There is usually non-specific laboratory evidence of inflammation at diagnosis but no diagnostic test. Normalized volumes from 89/889 2-D protein spots representing 26 proteins revealed a plasma pattern that distinguishes SJIA flare from quiescence. Highly discriminating spots derived from 15 proteins constitute a robust SJIA flare signature and show specificity for SJIA flare in comparison to active polyarticular juvenile idiopathic arthritis or acute febrile illness. We used 7 available ELISA assays, including one to the complex of S100A8/S100A9, to measure levels of 8 of the15 proteins. Validating our DIGE results, this ELISA panel correctly classified independent SJIA flare samples, and distinguished them from acute febrile illness. Notably, data using the panel suggest its ability to improve on erythrocyte sedimentation rate or C-reactive protein or S100A8/S100A9, either alone or in combination in SJIA F/Q discriminations. Our results also support the panel's potential clinical utility as a predictor of incipient flare (within 9 wk) in SJIA subjects with clinically inactive disease. Pathway analyses of the 15 proteins in the SJIA flare versus quiescence signature corroborate growing evidence for a key role for IL-1 at disease flare.