The potential for derivation of embryonic stem cells in vertebrates

An analysis of embryonic stem cell (ESC) derivation in vertebrates has revealed that the potential to form ESC is dependent on the setting aside of a pluripotent lineage from extraembryonic lineages early in development. Derivation of ESCs from all amniotes and also many lower vertebrates with that...

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Published inMolecular reproduction and development Vol. 73; no. 1; pp. 123 - 131
Main Authors Familari, Mary, Selwood, Lynne
Format Journal Article
LanguageEnglish
Published Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.01.2006
Wiley-Liss
Subjects
Amniota
Animals
Biological and medical sciences
Cattle
Cell Culture Techniques
Cell Differentiation
Cell Line
Chick Embryo
culture conditions
Culture Media
embryonic stem cells
epiblast
Fundamental and applied biological sciences. Psychology
General aspects
Mice
pluriblast
Serum
Stem Cells - cytology
Tissue Extracts
vertebrates
Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution
Zebrafish
Cell culture
Genetic drift
Vertebrata
culture conditions
Embryonic cell
Stem cell
embryonic stem cells
Biological marker
Review
pluriblast
epiblast
vertebrates
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Summary:An analysis of embryonic stem cell (ESC) derivation in vertebrates has revealed that the potential to form ESC is dependent on the setting aside of a pluripotent lineage from extraembryonic lineages early in development. Derivation of ESCs from all amniotes and also many lower vertebrates with that pattern of lineage allocation is thus predictable. Culture conditions during derivation in all groups share some similar characteristics, most of which are related to retaining potency coupled with extensive proliferative capacity. This in turn probably reflects the environment that maintains and causes the primordial germ cells (PGC) to proliferate in vivo. Hence culture usually involves feeder layers and serum or factors derived from them and the use of small clumps of pluriblast or epiblast cells instead of total dissociation, to facilitate cell‐cell signalling. Currently addition of FGF has proven to be important but that of LIF has not been fully explored. Mol. Reprod. Dev. © 2005 Wiley‐Liss, Inc.
Bibliography:ark:/67375/WNG-4S15K2KX-T
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ArticleID:MRD20376
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1040-452X
1098-2795
DOI:10.1002/mrd.20376