Effects of various flavonoids isolated from Scutellaria baicalensis roots on skin damage in acute UVB-irradiated hairless mice

• Published in: Journal of pharmacy and pharmacology Vol. 63; no. 12; pp. 1613 - 1623
• Main Authors: Kimura, Yoshiyuki, Sumiyoshi, Maho
• Format: Journal Article
• Language: English
• Published: HOBOKEN Wiley 01.12.2011
• Subjects: Administration, Oral; Animals; Dermis - drug effects; Dermis - radiation effects; Ear, External - pathology; Flavonoids - administration & dosage; Flavonoids - chemistry; Flavonoids - pharmacology; Life Sciences & Biomedicine; Magnetic Resonance Spectroscopy; Male; Matrix Metalloproteinases - metabolism; Mice; Mice, Hairless; Pharmacology & Pharmacy; Plant Roots - chemistry; Regional Blood Flow - drug effects; Science & Technology; Scutellaria baicalensis - chemistry; Skin - blood supply; Skin - pathology; Skin - radiation effects; Skin Aging - pathology; Spectrophotometry, Infrared; Spectrophotometry, Ultraviolet; Ultraviolet Rays; Vascular Endothelial Growth Factor A - metabolism; LIPID-PEROXIDATION; ANGIOGENESIS; VEGF; RADIX; RADIATION; OLIVE LEAF EXTRACT; MMP; ADHESION MOLECULE EXPRESSION; PLASMINOGEN-ACTIVATOR; ultraviolet B; RECEPTOR AGONIST PEPTIDE; Scutellaria baicalensis; GROWTH-FACTOR; flavonoids; TUMOR-GROWTH
• ISSN: 0022-3573; 2042-7158
• DOI: 10.1111/j.2042-7158.2011.01365.x

Objectives Solar ultraviolet (UV) radiation causes skin damage including increasing skin thickness, edema and flush. Scutellaria baicalensis roots have been traditionally used as a remedy for allergic inflammatory diseases in China and Japan. In this study, we examined the effects of four flavonoids isolated from these roots, namely 2',5,5',7-tetrahydroxy-6',8-dimethoxyflavone (1), skullcapflavone II (2), 2(S)-2',5,6',7-tetrahydroxyflavanone (3) and 2(R), 3(R)-2',3,5,6',7-pentahydroxyflavanone (4), on acute UVB irradiation-induced skin damage in hairless mice. Methods The four flavonoids were orally administered twice daily, at doses of 10 and 50 mg/kg, for 14 consecutive days. The UVB irradiation was performed at a dose of 200 mJ cm(-2) on days 7 and 8 after beginning oral administration of the four flavonoids. Key findings Compounds 1 and 4 prevented increases in skin thickness, levels of matrix metalloproteinases (MMP)-2 and MMP-9, and vascular endothelial growth factor (VEGF) induced by UVB irradiation. The other two flavonoids 2 and 3 had no effect. Conclusions Compounds 1 and 4 isolated from Scutellaria baicalensis roots may be useful for preventing skin inflammation induced by acute UVB irradiation.