Lipoteichoic acid from Lactobacillus plantarum elicits both the production of Interleukin-23p19 and suppression of pathogen-mediated Interleukin-10 in THP-1 cells

• Published in: FEMS immunology and medical microbiology Vol. 49; no. 2; pp. 205 - 214
• Main Authors: Kim, Han Geun, Gim, Min Geun, Kim, Joo Yun, Jin Hwang, Hyun, Ham, Min Seok, Lee, Jung Min, Hartung, Thomas, Park, Jung Woo, Han, Seung Hyun, Chung, Dae Kyun
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.03.2007; Blackwell Publishing Ltd; Blackwell; Oxford University Press
• Subjects: Biological and medical sciences; Cell Line; Cytokines; Dose-Response Relationship, Immunologic; Fundamental and applied biological sciences. Psychology; Gene Expression; Humans; Interleukin 10; Interleukin-10 - antagonists & inhibitors; Interleukin-10 - biosynthesis; Interleukin-23; Interleukin-23 Subunit p19 - biosynthesis; Lactic acid; Lactic acid bacteria; Lactobacillus plantarum; Lactobacillus plantarum - immunology; Lipopolysaccharides - immunology; Lipoteichoic acid; Microbiology; Monocytes - microbiology; Pathogens; Plant cells; RNA, Messenger - analysis; RNA, Messenger - genetics; Staphylococcus aureus; Staphylococcus aureus - immunology; Teichoic Acids - immunology; Tumor Necrosis Factor-alpha - biosynthesis; Tumor necrosis factor-α; Interleukin-10; Interleukin-23; Lipoteichoic acid; Lactic acid bacteria; Lactobacillus plantarum; Microbiology; Suppression; Cytokine; lnterleukin-10; Immunology; Interleukin 10; Interleukin 1; Bacteria; Lactobacillaceae
• ISSN: 0928-8244; 1574-695X; 2049-632X
• DOI: 10.1111/j.1574-695X.2006.00175.x

In this study, the stimulatory effects of different lactic acid bacteria strains, and their subcellular fractions, on the THP-1 cell line were evaluated. Lactobacillus plantarum was found in particular to induce high levels of IL-23p19 mRNA, but it moderately induced TNF-α production. IL-10 production was not entirely affected by L. plantarum stimulation. When subcellular fractions of L. plantarum were used to treat THP-1 cells, IL-23p19 mRNA expression was enhanced in a dose-responsive manner, specifically by lipoteichoic acid (LTA). The cotreatment of THP-1 cells by both L. plantarum and Staphylococcus aureus LTA resulted in decreased IL-10 production when compared with cells treated by S. aureus LTA alone. Taken together, these data suggest that LTA isolated from L. plantarum elicits stimulatory effects upon the expression of IL-23p19 and inhibitory effects on pathogen-mediated IL-10 production.