Single multiplex assay to identify simultaneously enteropathogenic, enteroaggregative, enterotoxigenic, enteroinvasive and Shiga toxin-producing Escherichia coli strains in Brazilian children
A multiplex PCR to differentiate typical and atypical enteropathogenic Escherichia coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic (ETEC), enteroinvasive E. coli (EIEC) and Shiga toxin-producing E. coli (STEC) strains was developed and evaluated. The targets selected for each group we...
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| Published in | FEMS microbiology letters Vol. 267; no. 2; pp. 145 - 150 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
Oxford, UK
Oxford, UK : Blackwell Publishing Ltd
01.02.2007
Blackwell Publishing Ltd Blackwell Oxford University Press |
| Subjects | |
| Online Access | Get full text |
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| Abstract | A multiplex PCR to differentiate typical and atypical enteropathogenic Escherichia coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic (ETEC), enteroinvasive E. coli (EIEC) and Shiga toxin-producing E. coli (STEC) strains was developed and evaluated. The targets selected for each group were eae and bfpA for EPEC, aggR for EAEC, elt and est for ETEC, ipaH for EIEC and stx for STEC isolates. This PCR was specific and sensitive for rapid detection of target isolates in stools. Among 79 children with acute diarrhea, this technique identified 13 (16.4%) with atypical EPEC, four (5%) with EAEC, three (3.8%) with typical EPEC, one (1.3%) with ETEC and one (1.3%) with EIEC. |
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| AbstractList | AbstractA multiplex PCR to differentiate typical and atypical enteropathogenic Escherichia coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic (ETEC), enteroinvasive E. coli (EIEC) and Shiga toxin-producing E. coli (STEC) strains was developed and evaluated. The targets selected for each group were eae and bfpA for EPEC, aggR for EAEC, elt and est for ETEC, ipaH for EIEC and stx for STEC isolates. This PCR was specific and sensitive for rapid detection of target isolates in stools. Among 79 children with acute diarrhea, this technique identified 13 (16.4%) with atypical EPEC, four (5%) with EAEC, three (3.8%) with typical EPEC, one (1.3%) with ETEC and one (1.3%) with EIEC. Abstract A multiplex PCR to differentiate typical and atypical enteropathogenic Escherichia coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic (ETEC), enteroinvasive E. coli (EIEC) and Shiga toxin-producing E. coli (STEC) strains was developed and evaluated. The targets selected for each group were eae and bfpA for EPEC, aggR for EAEC, elt and est for ETEC, ipaH for EIEC and stx for STEC isolates. This PCR was specific and sensitive for rapid detection of target isolates in stools. Among 79 children with acute diarrhea, this technique identified 13 (16.4%) with atypical EPEC, four (5%) with EAEC, three (3.8%) with typical EPEC, one (1.3%) with ETEC and one (1.3%) with EIEC. A multiplex PCR to differentiate typical and atypical enteropathogenic Escherichia coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic (ETEC), enteroinvasive E. coli (EIEC) and Shiga toxin-producing E. coli (STEC) strains was developed and evaluated. The targets selected for each group were eae and bfpA for EPEC, aggR for EAEC, elt and est for ETEC, ipaH for EIEC and stx for STEC isolates. This PCR was specific and sensitive for rapid detection of target isolates in stools. Among 79 children with acute diarrhea, this technique identified 13 (16.4%) with atypical EPEC, four (5%) with EAEC, three (3.8%) with typical EPEC, one (1.3%) with ETEC and one (1.3%) with EIEC. |
| Author | Aranda, Katia R.S Fabbricotti, Sandra H Scaletsky, Isabel C.A Fagundes-Neto, Ulysses |
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| Cites_doi | 10.1128/JCM.43.2.755-760.2005 10.1128/IAI.67.7.3410-3415.1999 10.1128/JCM.42.1.398-400.2004 10.1128/JCM.38.5.2001-2004.2000 10.3201/eid0808.010492 10.1128/JCM.40.4.1254-1258.2002 10.1128/JCM.42.2.903-905.2004 10.1128/JCM.42.12.5849-5853.2004 10.1128/JCM.41.6.2669-2671.2003 10.1073/pnas.87.20.7839 |
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| Copyright | 2007 Federation of European Microbiological Societies 2007 2007 INIST-CNRS 2007 Federation of European Microbiological Societies |
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| Keywords | multiplex PCR diarrhea Human Multiplex polymerase chain reaction Escherichia coli Diarrhea Identification Polymerase chain reaction Digestive diseases Bacteria Intestinal disease Child Shiga like toxin Enterobacteriaceae |
| Language | English |
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| References | 2004; 10 2004; 42 1990; 87 2000; 38 2002a; 40 2006; 44 2002b; 8 1999; 67 1985 2005; 43 1993; 168 2005; 49 1996; 27 2003; 41 1998; 11 10.1111/j.1574-6968.2006.00580.x-BIB16 10.1111/j.1574-6968.2006.00580.x-BIB17 10.1111/j.1574-6968.2006.00580.x-BIB11 10.1111/j.1574-6968.2006.00580.x-BIB12 10.1111/j.1574-6968.2006.00580.x-BIB8 10.1111/j.1574-6968.2006.00580.x-BIB13 10.1111/j.1574-6968.2006.00580.x-BIB9 10.1111/j.1574-6968.2006.00580.x-BIB14 10.1111/j.1574-6968.2006.00580.x-BIB6 10.1111/j.1574-6968.2006.00580.x-BIB7 10.1111/j.1574-6968.2006.00580.x-BIB4 10.1111/j.1574-6968.2006.00580.x-BIB5 10.1111/j.1574-6968.2006.00580.x-BIB10 10.1111/j.1574-6968.2006.00580.x-BIB2 10.1111/j.1574-6968.2006.00580.x-BIB3 Trabulsi (10.1111/j.1574-6968.2006.00580.x-BIB15) 1985 10.1111/j.1574-6968.2006.00580.x-BIB1 |
| References_xml | – volume: 42 start-page: 903 year: 2004 end-page: 905 article-title: Virulence properties and characteristics of Shiga toxin‐producing in Sao Paulo, Brazil, from 1976 through 1999 publication-title: J Clin Microbiol – volume: 67 start-page: 3410 year: 1999 end-page: 3415 article-title: A localized adherence‐like pattern as a second pattern of adherence of classic enteropathogenic to HEp‐2 cells that is associated with infantile diarrhea publication-title: Infect Immunol – volume: 8 start-page: 855 year: 2002b end-page: 858 article-title: HEp‐2 adherent strains associated with acute infantile diarrhea, Sao Paulo, Brazil publication-title: Emerg Infect Dis – start-page: 121 year: 1985 end-page: 125 – volume: 87 start-page: 7839 year: 1990 end-page: 7843 article-title: A genetic locus of enteropathogenic necessary for the production of attaching and effacing lesions on tissue culture cells publication-title: Proc Natl Acad Sci USA – volume: 40 start-page: 1254 year: 2002a end-page: 1258 article-title: Comparison of DNA hybridization and PCR assays for detection of putative pathogenic enteroadherent publication-title: J Clin Microbiol – volume: 27 start-page: 130 year: 1996 end-page: 133 article-title: Defining EPEC publication-title: Rev Microbiol – volume: 38 start-page: 2001 year: 2000 end-page: 2004 article-title: Multiplex PCR for identification of virulence genes publication-title: J Clin Microbiol – volume: 41 start-page: 2669 year: 2003 end-page: 2671 article-title: Multiplex PCR assay for identification of human diarrheagenic publication-title: J Clin Microbiol – volume: 42 start-page: 398 year: 2004 end-page: 400 article-title: Reduced etiological role for enteropathogenic in cases of diarrhea in Brazilian infants publication-title: J Clin Microbiol – volume: 43 start-page: 755 year: 2005 end-page: 760 article-title: Detection and characterization of diarrheagenic from young children in Hanoi, Vietnam publication-title: J Clin Microbiol – volume: 42 start-page: 5849 year: 2004 end-page: 5853 article-title: Evaluation of multiplex PCRs for diagnosis of infection with diarrheagenic and spp publication-title: J Clin Microbiol – volume: 10 start-page: 1851 year: 2004 end-page: 1855 article-title: Emerging enteropathogenic strains publication-title: Emerg Infect Dis – volume: 11 start-page: 142 year: 1998 end-page: 201 article-title: Diarrheagenic publication-title: Clin Microbiol Rev – volume: 49 start-page: 485 year: 2005 end-page: 492 article-title: Rapid categorization of pathogenic by multiplex PCR publication-title: Microbiol Immunol – volume: 168 start-page: 1037 year: 1993 end-page: 1041 article-title: Distribution of the bundle‐forming‐pilus structural gene ( ) among enteropathogenic publication-title: J Infect Dis – volume: 44 start-page: 2626 year: 2006 end-page: 2629 article-title: Rapid identification and differention of clinical isolates of enteropathogenic (EPEC), atypical EPEC, and shiga toxin‐producing by a one‐step multiplex PCR method publication-title: J Clin Microbiol – ident: 10.1111/j.1574-6968.2006.00580.x-BIB6 – ident: 10.1111/j.1574-6968.2006.00580.x-BIB7 – ident: 10.1111/j.1574-6968.2006.00580.x-BIB17 doi: 10.1128/JCM.43.2.755-760.2005 – ident: 10.1111/j.1574-6968.2006.00580.x-BIB8 – ident: 10.1111/j.1574-6968.2006.00580.x-BIB2 – ident: 10.1111/j.1574-6968.2006.00580.x-BIB5 – ident: 10.1111/j.1574-6968.2006.00580.x-BIB3 – ident: 10.1111/j.1574-6968.2006.00580.x-BIB11 doi: 10.1128/IAI.67.7.3410-3415.1999 – start-page: 121 volume-title: Infectious Diarrhea in the Young year: 1985 ident: 10.1111/j.1574-6968.2006.00580.x-BIB15 contributor: fullname: Trabulsi – ident: 10.1111/j.1574-6968.2006.00580.x-BIB10 doi: 10.1128/JCM.42.1.398-400.2004 – ident: 10.1111/j.1574-6968.2006.00580.x-BIB9 doi: 10.1128/JCM.38.5.2001-2004.2000 – ident: 10.1111/j.1574-6968.2006.00580.x-BIB13 doi: 10.3201/eid0808.010492 – ident: 10.1111/j.1574-6968.2006.00580.x-BIB12 doi: 10.1128/JCM.40.4.1254-1258.2002 – ident: 10.1111/j.1574-6968.2006.00580.x-BIB16 doi: 10.1128/JCM.42.2.903-905.2004 – ident: 10.1111/j.1574-6968.2006.00580.x-BIB1 doi: 10.1128/JCM.42.12.5849-5853.2004 – ident: 10.1111/j.1574-6968.2006.00580.x-BIB14 doi: 10.1128/JCM.41.6.2669-2671.2003 – ident: 10.1111/j.1574-6968.2006.00580.x-BIB4 doi: 10.1073/pnas.87.20.7839 |
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| Snippet | A multiplex PCR to differentiate typical and atypical enteropathogenic Escherichia coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic (ETEC),... Abstract A multiplex PCR to differentiate typical and atypical enteropathogenic Escherichia coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic... AbstractA multiplex PCR to differentiate typical and atypical enteropathogenic Escherichia coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic... |
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| SubjectTerms | Bacteria Bacterial Typing Techniques Bacteriology Biological and medical sciences Brazil Child, Preschool Children Diarrhea Diarrhea - microbiology E coli Escherichia coli Escherichia coli - classification Escherichia coli - isolation & purification Escherichia coli - pathogenicity Escherichia coli Infections - diagnosis Escherichia coli Infections - microbiology Fundamental and applied biological sciences. Psychology Humans Microbiology multiplex PCR Multiplexing Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Polymerase Chain Reaction - methods Sensitivity and Specificity Shiga toxin Shiga Toxin - metabolism Target detection Toxins |
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| Title | Single multiplex assay to identify simultaneously enteropathogenic, enteroaggregative, enterotoxigenic, enteroinvasive and Shiga toxin-producing Escherichia coli strains in Brazilian children |
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