Single multiplex assay to identify simultaneously enteropathogenic, enteroaggregative, enterotoxigenic, enteroinvasive and Shiga toxin-producing Escherichia coli strains in Brazilian children

• Published in: FEMS microbiology letters Vol. 267; no. 2; pp. 145 - 150
• Main Authors: Aranda, Katia R.S, Fabbricotti, Sandra H, Fagundes-Neto, Ulysses, Scaletsky, Isabel C.A
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.02.2007; Blackwell Publishing Ltd; Blackwell; Oxford University Press
• Subjects: Bacteria; Bacterial Typing Techniques; Bacteriology; Biological and medical sciences; Brazil; Child, Preschool; Children; Diarrhea; Diarrhea - microbiology; E coli; Escherichia coli; Escherichia coli - classification; Escherichia coli - isolation & purification; Escherichia coli - pathogenicity; Escherichia coli Infections - diagnosis; Escherichia coli Infections - microbiology; Fundamental and applied biological sciences. Psychology; Humans; Microbiology; multiplex PCR; Multiplexing; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Polymerase Chain Reaction - methods; Sensitivity and Specificity; Shiga toxin; Shiga Toxin - metabolism; Target detection; Toxins; South America; Brazil; America; multiplex PCR; diarrhea; Human; Multiplex polymerase chain reaction; Escherichia coli; Diarrhea; Identification; Polymerase chain reaction; Digestive diseases; Bacteria; Intestinal disease; Child; Shiga like toxin; Enterobacteriaceae
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.2006.00580.x

A multiplex PCR to differentiate typical and atypical enteropathogenic Escherichia coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic (ETEC), enteroinvasive E. coli (EIEC) and Shiga toxin-producing E. coli (STEC) strains was developed and evaluated. The targets selected for each group were eae and bfpA for EPEC, aggR for EAEC, elt and est for ETEC, ipaH for EIEC and stx for STEC isolates. This PCR was specific and sensitive for rapid detection of target isolates in stools. Among 79 children with acute diarrhea, this technique identified 13 (16.4%) with atypical EPEC, four (5%) with EAEC, three (3.8%) with typical EPEC, one (1.3%) with ETEC and one (1.3%) with EIEC.