Strain-specific salt tolerance and osmoregulatory mechanisms in Azospirillum brasilense

• Published in: FEMS microbiology letters Vol. 267; no. 1; pp. 72 - 79
• Main Authors: Chowdhury, Soumitra Paul, Nagarajan, Thirunavukkarasu, Tripathi, Rachna, Mishra, Mukti Nath, Le Rudulier, Daniel, Tripathi, Anil Kumar
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.02.2007; Blackwell Publishing Ltd; Blackwell; Oxford University Press
• Subjects: Abiotic stress; Acetylene; Acetylene - metabolism; Action of physical and chemical agents on bacteria; Adaptation, Physiological; Adhesins, Bacterial; Aggregates; aggregation; Anti-Bacterial Agents - pharmacology; Azospirillum brasilense; Azospirillum brasilense - classification; Azospirillum brasilense - genetics; Azospirillum brasilense - isolation & purification; Azospirillum brasilense - physiology; Bacteriology; Betaine - metabolism; Biological and medical sciences; Cell culture; Cereals; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Edible Grain - microbiology; exopolysaccharide production; Exopolysaccharides; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Glycine; Glycine betaine; India; Microbiology; Molecular Sequence Data; Nitrogen fixation; Nitrogenation; Osmolar Concentration; Osmoregulation; Osmosis; Oxidation-Reduction; Phylogeny; Plant growth; Poaceae - microbiology; Polysaccharides, Bacterial - biosynthesis; Restoration; Rhizosphere; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; rRNA 16S; Salt tolerance; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Sodium Chloride - pharmacology; Water-Electrolyte Balance; Yeast; India; salt tolerance; aggregation; exopolysaccharide production; nitrogen fixation; glycine betaine; Pseudomonadales; Spirillaceae; Azospirillum brasilense; Bacillaceae; Glycine; Pseudomonas; Mechanism; Aggregation; Salt; Enterobacter; Bacillales; Betaine; Bacillus; Nitrogen fixation; Bacteria; Pseudomonadaceae; Enterobacteriaceae; Spirillales; Phylogenetic tree; Tolerance; Strain; Neurotransmitter
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.2006.00540.x

Salinity stress inhibits the growth and nitrogen fixation ability of the plant growth-promoting rhizobacterium Azospirillum brasilense. Five strains of A. brasilense were isolated from the rhizosphere of Indian cereals and grasses and identified on the basis of their phenotypic features and 16S rRNA gene sequence. The five Indian isolates and two standard strains of A. brasilense, Sp7 and Cd, showed notable differences in growth, acetylene-reducing activity under salt stress, and ability to take up and use glycine betaine for the restoration of growth and acetylene-reducing activity under salt stress. Salt stress also enhanced the production of exopolysaccharides and cell aggregates, the extent of which varied in different strains of A. brasilense at different carbon to nitrogen ratios in the culture medium. It can be concluded that the production of exopolysaccharides and cell aggregates is a more consistent physiological response of A. brasilense to salt stress than is the uptake and osmoprotection by glycine betaine.