Glabridin synergy with norfloxacin induces ROS in multidrug resistant Staphylococcus aureus

Glabridin (Glb), a polyphenolic flavonoid inhibits the growth of MDRSA (Multidrug resistant S. aureus) 4627 by inducing ROS. Glb in combination with Norfloxacin (Nor) synergistically induced oxidative stress. Increased ROS/RNS levels, in particular, affected macromolecules' (DNA, lipid, protein...

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Published inJournal of general and applied microbiology Vol. 67; no. 6; pp. 269 - 272
Main Authors Pal, Anirban, Darokar, Mahendra P., Singh, Vigyasa
Format Journal Article
LanguageEnglish
Published Japan Applied Microbiology, Molecular and Cellular Biosciences Research Foundation 01.01.2021
Japan Science and Technology Agency
Subjects
Anti-Bacterial Agents - pharmacology
Antibacterial agents
Antiinfectives and antibacterials
Cell morphology
Cytology
drug resistance
Flavonoids
glabridin
Isoflavones
Lipids
Macromolecules
Methicillin-Resistant Staphylococcus aureus
Microbial Sensitivity Tests
Multidrug resistance
Norfloxacin
Norfloxacin - pharmacology
Oxidative stress
Phenols
Prospective Studies
Reactive Oxygen Species
S. aureus
Staphylococcus aureus
glabridin
S. aureus
drug resistance
norfloxacin
reactive oxygen species
Online AccessGet full text
ISSN0022-1260
1349-8037
DOI10.2323/jgam.2021.06.002

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Abstract Glabridin (Glb), a polyphenolic flavonoid inhibits the growth of MDRSA (Multidrug resistant S. aureus) 4627 by inducing ROS. Glb in combination with Norfloxacin (Nor) synergistically induced oxidative stress. Increased ROS/RNS levels, in particular, affected macromolecules' (DNA, lipid, protein) integrity and distorted cell morphology. We found correlation between drug-effects and up-/down-regulation of oxidative stress-related as well as MDR genes. These findings could considerably potentiate the dosing routine of Nor in combination with Glb, which holds a promising prospective as a antibacterial agent against S. aureus.
AbstractList Glabridin (Glb), a polyphenolic flavonoid inhibits the growth of MDRSA (Multidrug resistant S. aureus) 4627 by inducing ROS. Glb in combination with Norfloxacin (Nor) synergistically induced oxidative stress. Increased ROS/RNS levels, in particular, affected macromolecules' (DNA, lipid, protein) integrity and distorted cell morphology. We found correlation between drug-effects and up-/down-regulation of oxidative stress-related as well as MDR genes. These findings could considerably potentiate the dosing routine of Nor in combination with Glb, which holds a promising prospective as a antibacterial agent against S. aureus.
ArticleNumber 2021.06.002
Author Pal, Anirban
Singh, Vigyasa
Darokar, Mahendra P.
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  organization: Department of Pharmacology & Toxicology, College of Pharmacy, University of Arizona
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10.1038/nrmicro798
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10.1172/JCI38226
10.1016/j.freeradbiomed.2015.06.016
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reactive oxygen species
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Livermore, D. (2004) Can better prescribing turn the tide of resistance? Nat. Rev. Microbiol., 2, 73–78.
Fatima, A., Gupta, V. K., Luqman, S., Negi, A. S., Kumar, J. K. et al. (2009) Antifungal activity of Glycyrrhiza glabra extracts and its active constituent glabridin. Phytother. Res., 23, 1190–1193.
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Singh, V., Pal, A., and Darokar, M. P. (2015) A polyphenolic flavonoid glabridin: Oxidative stress response in multidrug-resistant Staphylococcus aureus. Free Radic Biol. Med., 87, 48–57.
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References_xml – reference: Li, N., Gao, C., Peng, X., Wang, W., Luo, M. et al. (2014) Aspidin BB, a phloroglucinol derivative, exerts its antibacterial activity against Staphylococcus aureus by inducing the generation of reactive oxygen species. Res. Microbiol., 165, 263–272.
– reference: DeLeo, F. R. and Chambers, H. F. (2009) Reemergence of antibiotic-resistant Staphylococcus aureus in the genomics era. J. Clin. Invest., 119, 2464–2474.
– reference: Farooq, S., Wahab, A. T., Fozing, C. D. A., Rahman, A. U., and Choudhary, M. I. (2014) Artonin I inhibits multidrug resistance in Staphylococcus aureus and potentiates the action of inactive antibiotics in vitro. J. App. Microbiol., 117, 996–1011.
– reference: Le, A., Cooper, C. R., Gouw, A. M., Dinavahi, R., Maitra, A. et al. (2010) Inhibition of lactate dehydrogenase A induces oxidative stress and inhibits tumor progression. Proc. Natl. Acad. Sci. USA, 107, 2037–2042.
– reference: Liochev, S. I. (2013) Reactive oxygen species and the free radical theory of aging. Free Radic. Biol. Med., 60, 1-4.
– reference: Livermore, D. (2004) Can better prescribing turn the tide of resistance? Nat. Rev. Microbiol., 2, 73–78.
– reference: Singh, V., Pal, A., and Darokar, M. P. (2015) A polyphenolic flavonoid glabridin: Oxidative stress response in multidrug-resistant Staphylococcus aureus. Free Radic Biol. Med., 87, 48–57.
– reference: Applerot, G., Lipovsky, A., Dror, R., Perkas, N., Nitzan, Y. et al. (2009) Enhanced antibacterial activity of nanocrystalline ZnO due to increased ROS-mediated cell injury. Adv. Funct. Mater., 19, 842–852.
– reference: Fatima, A., Gupta, V. K., Luqman, S., Negi, A. S., Kumar, J. K. et al. (2009) Antifungal activity of Glycyrrhiza glabra extracts and its active constituent glabridin. Phytother. Res., 23, 1190–1193.
– reference: Keyer, K. and Imlay, J. A. (1996) Superoxide accelerates DNA damage by elevating free-iron levels. Proc. Natl. Acad. Sci. USA, 93, 13635–13640.
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SubjectTerms Anti-Bacterial Agents - pharmacology
Antibacterial agents
Antiinfectives and antibacterials
Cell morphology
Cytology
drug resistance
Flavonoids
glabridin
Isoflavones
Lipids
Macromolecules
Methicillin-Resistant Staphylococcus aureus
Microbial Sensitivity Tests
Multidrug resistance
Norfloxacin
Norfloxacin - pharmacology
Oxidative stress
Phenols
Prospective Studies
Reactive Oxygen Species
S. aureus
Staphylococcus aureus
Title Glabridin synergy with norfloxacin induces ROS in multidrug resistant Staphylococcus aureus
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