An ELISA optimized for porcine epidemic diarrhoea virus detection in faeces

• Published in: Veterinary microbiology Vol. 105; no. 1; pp. 9 - 17
• Main Authors: RODAK, L, VALICEK, L, SMID, B, NEVORANKOVA, Z
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 05.01.2005; Elsevier Science
• Subjects: Animals; Antibodies, Blocking - immunology; Antibodies, Monoclonal - immunology; Applied microbiology; Biological and medical sciences; Blocking ELISA; Coronavirus - isolation & purification; Coronavirus Infections - diagnosis; Coronavirus Infections - veterinary; Coronavirus Infections - virology; Diarrhea - diagnosis; Diarrhea - veterinary; Diarrhea - virology; Electrophoresis, Polyacrylamide Gel - veterinary; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - veterinary; Feces - virology; Fundamental and applied biological sciences. Psychology; gastroenteritis virus; Immunoenzyme Techniques - veterinary; Microbiology; Microscopy, Electron - veterinary; Miscellaneous; Monoclonal antibodies; Porcine epidemic diarrhoea virus (PEDV); Rotavirus; Sensitivity and Specificity; Swine; Swine Diseases - diagnosis; Swine Diseases - virology; Transmissible gastroenteritis virus; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Viral gastroenteritis of swine; Viral Matrix Proteins - immunology; Virology; Monoclonal antibodies; Blocking ELISA; Viral gastroenteritis of swine; Porcine epidemic diarrhoea virus (PEDV); Porcine epidemic diarrhea virus; Microbiology; Gastroenteritis; Monoclonal antibody; Virus; Vertebrata; Coronavirus; Mammalia; Swine; Digestive diseases; Intestinal disease; Artiodactyla; Coronaviridae; Detection; Veterinary; ELISA assay; Nidovirales; Ungulata; Gastric disease
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2004.09.020

Monoclonal antibodies to porcine epidemic diarrhoea virus (PEDV) membrane protein M were prepared and used for the comparative assessment of three blocking ELISA variants to detect PEDV. The competitive blocking ELISA (CB-ELISA) format showed the highest sensitivity, allowing detection of 10 2.5 plaque-forming units of PEDV/ml in culture medium. Its specificity was verified by inclusion of control samples containing transmissible gastroenteritis virus (TGEV) and rotavirus A in each analysis. Eighty porcine field samples of faeces obtained from 38 herds affected with diarrhoea were examined, and PEDV was found in 15 (19%) samples from 6 (16%) herds. The suitability of the CB-ELISA for the screening herds in epizootiologic situations is discussed.