Expression of Major Lipid Raft Protein Raftlin in Chronic Rhinosinusitis with Nasal Polyps in Smoking and Non-Smoking Patients Correlated with Interleukin-17 and Tumor Necrosis Factor-α Levels

• Published in: Biomolecules (Basel, Switzerland) Vol. 12; no. 9; p. 1316
• Main Authors: Lin, Yu-Tsai, Tsai, Ming-Hsien, Su, Yan-Ye, Chen, Wei-Chih, Huang, Shun-Chen, Chien, Chih-Yen
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.09.2022; MDPI
• Subjects: Antibodies; Biomarkers; Cellular proteins; chronic rhinosinusitis; Cigarette smoking; Cytokines; Enzyme-linked immunosorbent assay; Epithelium; Health aspects; Inflammation; Inflammatory diseases; Interleukin 17; Laboratories; Lipids; Methods; Nasal polyps; Necrosis; Nose; Otolaryngology; Patients; Physiological aspects; Polyps; Raftlin; Rhinitis; Rhinosinusitis; Sepsis; Signal transduction; Sinusitis; Sleep apnea; Smokers; Smoking; Software; Statistical analysis; Surgery; Tumor necrosis factor; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Variance analysis; Taiwan; United States > US
• ISSN: 2218-273X; 2218-273X
• DOI: 10.3390/biom12091316

Raftlin, as an inflammatory biomarker, has been previously reported in chronic inflammatory diseases. This study investigates the expression of Raftlin in cigarette smokers and in chronic rhinosinusitis with nasal polyps (CRSwNP), as well as evaluating its correlation with interleukin-17 (IL-17) and tumor necrosis factor-α (TNF-α) levels. A total of 30 CRSwNP non-smoking and 16 CRSwNP + SK (smoking) patients undergoing endoscopic sinus surgery were enrolled, while 20 middle turbinate tissue pieces were examined and performed as the control group. In nasal mucosa epithelial staining, Raftlin levels were elevated in the columnar cells and were stained much more intensely in the CRSwNP and CRSwNP + SK groups. Raftlin was located more closely to the apical region of the epithelium in the CRSwNP + SK group; however, the Raftlin levels from whole nasal tissue pieces, according to ELISA data, showed that there was no significant difference between the three different study groups. A positive relationship by Pearson correlation was found between IL-17 or TNF-α levels and Raftlin levels. Taken together, these data indicate that increasing Raftlin expression in columnar cells might involve nasal epithelial remodeling in smokers with CRSwNP.