CRISPR/Cas9 systems: Delivery technologies and biomedical applications

• Published in: Asian journal of pharmceutical sciences Vol. 18; no. 6; p. 100854
• Main Authors: Du, Yimin, Liu, Yanfei, Hu, Jiaxin, Peng, Xingxing, Liu, Zhenbao
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2023; Shenyang Pharmaceutical University; Elsevier
• Subjects: Biomedical applications; CRISPR/Cas9; Non-viral vector; Physical delivery; Review; Viral vector; Physical delivery; Non-viral vector; Biomedical applications; CRISPR/Cas9; Viral vector
• ISSN: 1818-0876; 2221-285X; 2221-285X
• DOI: 10.1016/j.ajps.2023.100854

The emergence of the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) genome-editing system has brought about a significant revolution in the realm of managing human diseases, establishing animal models, and so on. To fully harness the potential of this potent gene-editing tool, ensuring efficient and secure delivery to the target site is paramount. Consequently, developing effective delivery methods for the CRISPR/Cas9 system has become a critical area of research. In this review, we present a comprehensive outline of delivery strategies and discuss their biomedical applications in the CRISPR/Cas9 system. We also provide an in-depth analysis of physical, viral vector, and non-viral vector delivery strategies, including plasmid-, mRNA- and protein-based approach. In addition, we illustrate the biomedical applications of the CRISPR/Cas9 system. This review highlights the key factors affecting the delivery process and the current challenges facing the CRISPR/Cas9 system, while also delineating future directions and prospects that could inspire innovative delivery strategies. This review aims to provide new insights and ideas for advancing CRISPR/Cas9-based delivery strategies and to facilitate breakthroughs in biomedical research and therapeutic applications. This review provided an overview of the current state-of-the-art delivery strategies for CRISPR/Cas9 systems and their biomedical applications. The challenges, new insights, and future directions for their development were proposed. [Display omitted]