SMART-LAMP: A Smartphone-Operated Handheld Device for Real-Time Colorimetric Point-of-Care Diagnosis of Infectious Diseases via Loop-Mediated Isothermal Amplification

Nucleic acid amplification diagnostics offer outstanding features of sensitivity and specificity. However, they still lack speed and robustness, require extensive infrastructure, and are neither affordable nor user-friendly. Thus, they have not been extensively applied in point-of-care diagnostics,...

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Published inBiosensors (Basel) Vol. 12; no. 6; p. 424
Main Authors García-Bernalt Diego, Juan, Fernández-Soto, Pedro, Márquez-Sánchez, Sergio, Santos Santos, Daniel, Febrer-Sendra, Begoña, Crego-Vicente, Beatriz, Muñoz-Bellido, Juan Luis, Belhassen-García, Moncef, Corchado Rodríguez, Juan M, Muro, Antonio
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.06.2022
MDPI
Subjects
Analysis
Bluetooth
Colorimetry
Communicable diseases
Coronaviruses
COVID-19
Diagnosis
Disease transmission
Gene amplification
Infectious diseases
infectious diseases SARS-CoV-2
Infrastructure
loop-mediated isothermal amplification
Methods
mHealth
Neglected Tropical Diseases
Nucleic acids
Patients
point-of-care diagnostics
Portable equipment
Real time
Room temperature
Schistosomiasis
Severe acute respiratory syndrome coronavirus 2
Smart phones
Smartphones
Strongyloidiasis
Telemedicine
Tropical diseases
Germany
Spain
United Kingdom > UK
United States > US
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Summary:Nucleic acid amplification diagnostics offer outstanding features of sensitivity and specificity. However, they still lack speed and robustness, require extensive infrastructure, and are neither affordable nor user-friendly. Thus, they have not been extensively applied in point-of-care diagnostics, particularly in low-resource settings. In this work, we have combined the loop-mediated isothermal amplification (LAMP) technology with a handheld portable device (SMART-LAMP) developed to perform real-time isothermal nucleic acid amplification reactions, based on simple colorimetric measurements, all of which are Bluetooth-controlled by a dedicated smartphone app. We have validated its diagnostic utility regarding different infectious diseases, including Schistosomiasis, Strongyloidiasis, and COVID-19, and analyzed clinical samples from suspected COVID-19 patients. Finally, we have proved that the combination of long-term stabilized LAMP master mixes, stored and transported at room temperature with our developed SMART-LAMP device, provides an improvement towards true point-of-care diagnosis of infectious diseases in settings with limited infrastructure. Our proposal could be easily adapted to the diagnosis of other infectious diseases.
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ISSN:2079-6374
2079-6374
DOI:10.3390/bios12060424