Isolation of a novel platform bacterium for lignin valorization and its application in glucose-free cis,cis-muconate production

• Published in: Journal of industrial microbiology & biotechnology Vol. 46; no. 8; pp. 1071 - 1080
• Main Authors: Shinoda, Eri, Takahashi, Kenji, Abe, Nanase, Kamimura, Naofumi, Sonoki, Tomonori, Masai, Eiji
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.08.2019; Oxford University Press
• Subjects: Biochemistry; Bioinformatics; Biomedical and Life Sciences; Biotechnology; Catechol; Catechol 1,2-dioxygenase; Catechols - metabolism; Chemical compounds; Depolymerization; Energy sources; Flavin; Genes; Genetic Engineering; Glucose; Glucose - metabolism; Inorganic Chemistry; Intramolecular Lyases - metabolism; Life Sciences; Lignin; Lignin - metabolism; Metabolic Engineering and Synthetic Biology - Original Paper; Microbiology; Microorganisms; Organic chemistry; Oxidoreductases, O-Demethylating - metabolism; Phenolic compounds; Phenols; Plasmids - genetics; Pseudomonas - genetics; Pseudomonas - isolation & purification; Pseudomonas - metabolism; Sorbic Acid - analogs & derivatives; Sorbic Acid - metabolism; Syringate; Vanillate O-demethylase; Lignin valorization; ,; Muconate; Pseudomonas; cis,cis-Muconate
• ISSN: 1367-5435; 1476-5535
• DOI: 10.1007/s10295-019-02190-6

Microbial production of cis , cis -muconate (ccMA) from phenolic compounds obtained by chemical depolymerization of lignin is a promising approach to valorize lignin. Because microbial production requires a large amount of carbon and energy source, it is desirable to establish a ccMA-producing strain that utilizes lignin-derived phenols instead of general sources like glucose. We isolated Pseudomonas sp. strain NGC7 that grows well on various phenolic compounds derived from p -hydroxyphenyl, guaiacyl, and syringyl units of lignin. An NGC7 mutant of protocatechuate (PCA) 3,4-dioxygenase and ccMA cycloisomerase genes (NGC703) lost the ability to grow on vanillate and p -hydroxybenzoate but grew normally on syringate. Introduction of a plasmid carrying genes encoding PCA decarboxylase, flavin prenyltransferase, vanillate O -demethylase, and catechol 1,2-dioxygenase into NGC703 enabled production of 3.2 g/L ccMA from vanillate with a yield of 75% while growing on syringate. This strain also produced ccMA from birch lignin-derived phenols. All these results indicate the utility of NGC7 in glucose-free ccMA production.