Isolation and inheritance of microsatellite markers in the common carp Cyprinus carpio

Three microsatellite loci, Cca-17 super(*), Cca-21 super(*), and Cca-30 super(*), were isolated from a partial genome library of common carp Cyprinus carpio. Primers for PCR amplification of template DNA were designed based on the unique sequences flanking each motif. The total number of alleles det...

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Published inFisheries science Vol. 65; no. 2; pp. 235 - 239
Main Authors Aliah, R.S. (Kochi Univ., Nankoku (Japan). Faculty of Agriculture), Takagi, M, Dong, S, Teoh, C.T, Taniguchi, N
Format Journal Article
LanguageEnglish
Published 1999
Subjects
ANALYTICAL METHODS
CYPRINUS CARPIO
Freshwater
GENETIC INHERITANCE
HEREDITE
HERENCIA GENETICA
MICROSATELITES
MICROSATELLITE
MICROSATELLITES
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
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Summary:Three microsatellite loci, Cca-17 super(*), Cca-21 super(*), and Cca-30 super(*), were isolated from a partial genome library of common carp Cyprinus carpio. Primers for PCR amplification of template DNA were designed based on the unique sequences flanking each motif. The total number of alleles detected from nishikigoi and wild type common carp population were five, six and nine at loci Cca-17 super(*), Cca-21 super(*), and Cca-30 super(*), respectively. Inheritance was shown to be Mendelian by analysis of genotype ratio in F1 off-spring of six families of nishikigoi. Allele super(*)69 at locus Cca-21 super(*) and allele super(*)266 at locus Cca-30 super(*) were detected only in wild type common carp with frequencies of 0.304 and 0.458, respectively, while allele super(*)288 was observed only in the nishikigoi with the frequencies ranged from 0.600-0.610.
Bibliography:L10
1999006545
ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0919-9268
1444-2906
DOI:10.2331/fishsci.65.235