Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial DNA

The main objective of this study was to estimate the genetic diversity levels and haplotype traceability in pacu Piaractus mesopotamicus from the breeding program located in Brazil by analyses of the mitochondrial DNA control region (mtDNA). Moreover, broodstocks from eight commercial fish farms wer...

Full description

Saved in:
Bibliographic Details
Published inGenetics and molecular biology Vol. 44; no. 1; p. e20200249
Main Authors Freitas, Milena V de, Ariede, Raquel B, Hata, Milene E, Mastrochirico-Filho, Vito A, Pazo, Felipe Del, Villanova, Gabriela V, Mendonça, Fernando F, Porto-Foresti, Fábio, Hashimoto, Diogo T
Format Journal Article
LanguageEnglish
Published Brazil Sociedade Brasileira de Genética 01.01.2021
Subjects
Animal Genetics
aquaculture
BIOCHEMISTRY & MOLECULAR BIOLOGY
Control-region
genetic breeding
GENETICS & HEREDITY
genetic breeding
aquaculture
Control-region
Online AccessGet full text

Cover

More Information
Summary:The main objective of this study was to estimate the genetic diversity levels and haplotype traceability in pacu Piaractus mesopotamicus from the breeding program located in Brazil by analyses of the mitochondrial DNA control region (mtDNA). Moreover, broodstocks from eight commercial fish farms were used for comparative evaluation, four from Brazil (Br1-Br4) and four from Argentina (Ar1-Ar4). The descriptive results revealed 47 polymorphic sites and 51 mutations, which evidenced 34 haplotypes. Ten haplotypes were shared among fish farms and 24 were exclusive. The nucleotide diversity (π) ranged from 0.00031 to 0.01462 and haplotype diversity (Hd) from 0.125 to 0.868. The analysis of molecular variance (AMOVA) indicated high structure present in the analyzed stocks (FST = 0.13356 and ФST = 0.52707). The genetic diversity was high in most of the commercial broodstocks, especially those from Brazil. We observed seven haplotypes in the genetic breeding population, of which four were exclusive and three shared among the commercial fish farms. The genetic diversity was moderate (π = 0.00265 and Hd = 0.424) and considered appropriated for this breeding population of pacu. Our results provide support for the genetic diversity maintenance and mtDNA traceability of pacu commercial broodstocks.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Associate Editor: Vera Maria Fonseca de Almeida e Val
Author Contributions: MVF and DTH conceived and the study, MVF, RBA, MEH, VAMF and FP conducted the experiments, MVF, FP, GV, FFM and FPF analyzed the data, MVF and DTH wrote the manuscript. All authors read and approved the final version.
Conflict of Interest: The authors declare that there is no conflict of interest that could be perceived as prejudicial to the impartiality of the reported research.
ISSN:1415-4757
1678-4685
1678-4685
DOI:10.1590/1678-4685-GMB-2020-0249