Integrating spatially resolved three-dimensional MALDI IMS with in vivo magnetic resonance imaging

We have developed a method for integrating three dimensional-volume reconstructions of spatially resolved matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) ion images of whole mouse heads with high-resolution images from other modalities in an animal-specific manner....

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Bibliographic Details
Published inNature methods Vol. 5; no. 1; pp. 57 - 59
Main Authors Sinha, Tuhin K, Khatib-Shahidi, Sheerin, Yankeelov, Thomas E, Mapara, Khubaib, Ehtesham, Moneeb, Cornett, D Shannon, Dawant, Benoit M, Caprioli, Richard M, Gore, John C
Format Journal Article
LanguageEnglish
Published United States Nature Publishing Group 01.01.2008
Subjects
Animals
Brain - anatomy & histology
Brain - metabolism
Desorption
Imaging, Three-Dimensional - methods
Ionization
Lasers
Magnetic resonance imaging
Magnetic Resonance Imaging - methods
Mass spectrometry
Mice
NMR
Nuclear magnetic resonance
Peptide Mapping - methods
Physiological aspects
Proteins
Proteome - metabolism
Proteomics
Research methodology
Rodents
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Systems Integration
Three dimensional imaging
Tissue Distribution
United States
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Summary:We have developed a method for integrating three dimensional-volume reconstructions of spatially resolved matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) ion images of whole mouse heads with high-resolution images from other modalities in an animal-specific manner. This approach enabled us to analyze proteomic profiles from MALDI IMS data with corresponding in vivo data provided by magnetic resonance imaging.
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AUTHOR CONTRIBUTIONS
T.K.S. helped to develop the techniques presented here and assisted in collecting and analyzing the results. S.K.-S. helped to acquire the blockface and MALDI IMS data. T.E.Y. helped to acquire the in vivo magnetic resonance data. K.M. implanted and provided the mouse with a tumor-laden brain. M.E. provided support and expertise with the tumor model. D.S.C. provided expertise in collecting the MALDI IMS data. B.M.D. helped to develop accurate coregistration techniques for the MALDI IMS and magnetic resonance alignment. R.M.C. provided support and expertise for the MALDI IMS data collection. J.C.G. helped to develop the techniques presented here and provided expertise with data analysis and magnetic resonance data collection.
ISSN:1548-7091
1548-7105
DOI:10.1038/nmeth1147