Cytochrome P450 monooxygenases involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium

• Published in: Applied microbiology and biotechnology Vol. 87; no. 5; pp. 1907 - 1916
• Main Authors: Chigu, Nomathemba Loice, Hirosue, Sinji, Nakamura, Chie, Teramoto, Hiroshi, Ichinose, Hirofumi, Wariishi, Hiroyuki
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.08.2010; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Anthracene; Anthracenes - metabolism; Anthraquinones - metabolism; Applied Microbial and Cell Physiology; Basidiomycetes; Biodegradation; Biological and medical sciences; Biomedical and Life Sciences; Bioremediation; Biotechnology; Catalysis; Cytochrome; cytochrome P-450; Cytochrome P-450 Enzyme System - metabolism; Enzymes; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Profiling; Genomes; Life Sciences; Lignin; Metabolic Networks and Pathways; Metabolism; Microbial Genetics and Genomics; Microbiology; Mixed Function Oxygenases - metabolism; Oligonucleotide Array Sequence Analysis; Phanerochaete - enzymology; Phanerochaete - metabolism; Phanerochaete chrysosporium; Pollutants; Studies; substrate specificity; Transcriptional regulation; White-rot basidiomycete; Wood; White-rot basidiomycete; Bioremediation; Transcriptional regulation; Cytochrome P450; Substrate specificity; Fungi; Anthracene; Regulation(control); Transcription; Phanerochaete chrysosporium; Metabolism; Basidiomycota
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-010-2616-1

Cytochrome P450 monooxygenases (P450s) involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium were identified by comprehensive screening of both catalytic potentials and transcriptomic profiling. Functional screening of P. chrysosporium P450s (PcCYPs) revealed that 14 PcCYP species catalyze stepwise conversion of anthracene to anthraquinone via intermediate formation of anthrone. Moreover, transcriptomic profiling explored using a complementary DNA microarray system demonstrated that 12 PcCYPs are up-regulated in response to exogenous addition of anthracene. Among the up-regulated PcCYPs, five species showed catalytic activity against anthracene. Based upon both catalytic and transcriptional properties, these five species are most likely to play major roles in anthracene metabolic processes in vivo. Thus, the combination of functional screening and a microarray system may provide a novel strategy for obtaining a thorough understanding of the catalytic functions and biological impacts of PcCYPs.