Polyadenylation of Maternal RNA of Sea Urchin Eggs after Fertilization

Between fertilization, or parthenogenetic activation, and the two-cell stage, the content of polyadenylic acid in sea urchin eggs doubles, and the increase occurs primarily in the ribosome-polyribosome fraction. The increase is due to polyadenylation of preexisting RNA molecules synthesized during o...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 70; no. 8; pp. 2345 - 2349
Main Author Wilt, Fred H.
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences of the United States of America 01.08.1973
National Acad Sciences
Subjects
Actinomycin
Adenine Nucleotides - analysis
Adenine Nucleotides - metabolism
Adenosine - metabolism
Animals
Biological Sciences: Zoology
Cell Fractionation
Centrifugation, Density Gradient
Cytoplasm - metabolism
Dactinomycin - pharmacology
Eggs
Embryos
Female
Fertilization
Molecules
Oogenesis
Ova
Ovum - cytology
Ovum - metabolism
Polyadenylation
Polynucleotides - analysis
Polynucleotides - metabolism
RNA
RNA - analysis
RNA - isolation & purification
RNA - metabolism
Sea Urchins - metabolism
Sea water
Space life sciences
Tritium
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Summary:Between fertilization, or parthenogenetic activation, and the two-cell stage, the content of polyadenylic acid in sea urchin eggs doubles, and the increase occurs primarily in the ribosome-polyribosome fraction. The increase is due to polyadenylation of preexisting RNA molecules synthesized during oogenesis. The polyadenylation occurs in activated, enucleated merogons. It is argued that cytoplasmic polyadenylation may play a role in mobilization of maternal messenger RNA for translation and the polyadenylic acid does not subserve an exclusively nuclear function.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.70.8.2345