Immunoglobulin Enhancer and Promoter Motifs 5′ of the B29 B-Cell-Specific Gene

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 86; no. 19; pp. 7341 - 7345
• Main Authors: Hermanson, Gary G., Briskin, Michael, Sigman, David, Wall, Randolph
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.10.1989; National Acad Sciences
• Subjects: Animals; B lymphocytes; B-Lymphocytes - immunology; Base Sequence; Biological and medical sciences; Enhancer Elements, Genetic; Fundamental and applied biological sciences. Psychology; Genes; Genes, Immunoglobulin; Immunoglobulin genes; Immunoglobulin Heavy Chains - genetics; Immunoglobulin Variable Region - genetics; Immunoglobulins; Messenger RNA; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Multigene Family; Nucleotide Mapping; Nucleotide sequences; Nucleotides; Oligonucleotide Probes; Promoter regions; Promoter Regions, Genetic; RNA; RNA, Messenger - genetics; TATA box; Transcription, Genetic; Transcription. Transcription factor. Splicing. Rna processing; Molecular hybridization; Immunoglobulins; Gene; Gel electrophoresis; Tissue specificity; Transcription initiation; Lymphocyte
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.86.19.7341

B29 is a B-cell-specific member of the immunoglobulin gene superfamily that is expressed throughout B-cell development beginning with the earliest precursor B cells undergoing immunoglobulin heavy-chain gene segment rearrangements. We have analyzed the region upstream of the B29 gene to identify DNA sequences involved in transcriptional regulation of this gene. The B29 gene lacks a TATA box and transcription is initiated at multiple sites. The B29 gene sequence 5′ of these transcription start sites contains six promoter and enhancer motifs known to control immunoglobulin gene transcription. The most notable is a perfect octamer (5′-ATTTGCAT-3′), which binds the Oct-2 B-cell-specific transcription factor and thereby can account for the tissue-specific expression of this gene.