Dengue-2-virus-interacting polypeptides involved in mosquito cell infection

• Published in: Archives of virology Vol. 155; no. 9; pp. 1453 - 1461
• Main Authors: Paingankar, Mandar S, Gokhale, Mangesh D, Deobagkar, Dileep N
• Format: Journal Article
• Language: English
• Published: Vienna Vienna : Springer Vienna 01.09.2010; Springer Vienna; Springer; Springer Nature B.V
• Subjects: Aedes - genetics; Aedes - metabolism; Aedes - virology; Aedes aegypti; Aedes albopictus; Animals; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Cell Line; Dengue; Dengue fever; Dengue Virus - genetics; Dengue Virus - physiology; Dengue virus type 2; Exocrine glands; Fundamental and applied biological sciences. Psychology; Glycoproteins; Infections; Infectious Diseases; Insect Proteins - genetics; Insect Proteins - metabolism; Insect Vectors - genetics; Insect Vectors - metabolism; Insect Vectors - virology; Medical Microbiology; Microbiology; Miscellaneous; Molecular Sequence Data; Mosquitoes; Original Article; Peptides - genetics; Peptides - metabolism; Polypeptides; Proteins; Proteomics; Virology; Virulence; Virus Internalization; Zoology; India; Dengue Hemorrhagic Fever; Salivary Gland Extract; West Nile Virus; Mosquito Cell; Heat Shock Cognate; Virus; Dengue virus 2; Flaviviridae; Polypeptide; Flavivirus; Dengue group virus
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-010-0728-7

For the design of effective antiviral strategies, understanding the fundamental steps of the virus life cycle, including virus-host interactions, is essential. We performed a virus overlay protein binding assay followed by proteomics for identification of proteins from membrane fractions of A7 (Aedes aegypti) cells, C6/36 (Aedes albopictus) cells and the midgut brush border membrane fraction of Ae. aegypti mosquito that bind to dengue-2 virus. Actin, ATP synthase β subunit, HSc 70, orisis, prohibitin, tubulin β chain, and vav-1 were identified as dengue-2-virus-binding proteins. Our results suggest that dengue-2 virus exploits an array of housekeeping proteins for its entry in mosquito cells.