Protocol for screening facultative parthenogenesis in Drosophila

Most species of sexually reproducing Drosophila are capable of some degree of facultative parthenogenesis, which involves the initiation of development in an unfertilized egg. Here, we present an optimized protocol to screen facultative parthenogenesis in Drosophila. We describe steps for the collec...

Full description

Saved in:
Bibliographic Details
Published inSTAR protocols Vol. 4; no. 4; p. 102585
Main Authors Sperling, Alexis L., Glover, David M.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 15.12.2023
Elsevier
Subjects
Animals
Drosophila
Evolutionary Biology
Genetics
Model Organisms
Parthenogenesis
Protocol
Evolutionary Biology
Genetics
Model Organisms
Online AccessGet full text

Cover

More Information
Summary:Most species of sexually reproducing Drosophila are capable of some degree of facultative parthenogenesis, which involves the initiation of development in an unfertilized egg. Here, we present an optimized protocol to screen facultative parthenogenesis in Drosophila. We describe steps for the collection and maintenance of virgin flies. We then detail offspring screening for the analysis of parthenogenesis. This protocol can be applied to different Drosophila strains and can be adapted for the analysis of parthenogenesis in other animals. For complete details on the use and execution of this protocol, please refer to Sperling et al.1 [Display omitted] •A protocol for parthenogenesis screening in Drosophila•Screening genetic variants in Drosophila melanogaster for parthenogenesis•Temperature optimization for parthenogenesis screening•Control selection for parthenogenesis screening Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Most species of sexually reproducing Drosophila are capable of some degree of facultative parthenogenesis, which involves the initiation of development in an unfertilized egg. Here, we present an optimized protocol to screen facultative parthenogenesis in Drosophila. We describe steps for the collection and maintenance of virgin flies. We then detail offspring screening for the analysis of parthenogenesis. This protocol can be applied to different Drosophila strains and can be adapted for the analysis of parthenogenesis in other animals.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Technical contact
Lead contact
ISSN:2666-1667
2666-1667
DOI:10.1016/j.xpro.2023.102585