Enhancing the osteoinductive properties of hydroxyapatite by the addition of human mesenchymal stem cells, and recombinant human osteogenic protein-1 (BMP-7) in vitro

• Published in: Injury Vol. 37; no. 3; pp. S25 - S32
• Main Authors: Tsiridis, Eleftherios, Bhalla, Amit, Ali, Zubier, Gurav, Neelam, Heliotis, Manolis, Deb, Sanjukta, DiSilvio, Lucy
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Oxford Elsevier Ltd 01.09.2006; Elsevier
• Subjects: Adult; Anesthesia; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Biocompatible Materials - pharmacology; Biological and medical sciences; BMP-7; Bone graft; Bone Morphogenetic Protein 7; Bone Morphogenetic Proteins - physiology; Bone Regeneration - physiology; Cell Differentiation; Cell differentiation, maturation, development, hematopoiesis; Cell physiology; Cell Proliferation; Diseases of the osteoarticular system; Durapatite - pharmacology; Fundamental and applied biological sciences. Psychology; General anesthesia. Technics. Complications. Neuromuscular blocking. Premedication. Surgical preparation. Sedation; Humans; Hydroxyapatite; Male; Medical sciences; Mesenchymal stem cells; Mesenchymal Stromal Cells; Molecular and cellular biology; OP-1; Osteoblasts - physiology; Osteoinduction; Tissue Engineering - methods; OP-1; BMP-7; Hydroxyapatite; Bone graft; Osteoinduction; Mesenchymal stem cells; Human; Cell proliferation; Scanning electron microscopy; Incorporation; Blue; Stem cell; Cell differentiation; In vitro; Assay; Regulation(control); Bone morphogenetic protein; DNA; Anesthesia; Load cell; Recombinant protein; Comparative study; Bone morphogenetic protein 7; Bone substitute
• ISSN: 0020-1383; 1879-0267
• DOI: 10.1016/j.injury.2006.08.021

Hydroxyapatite (HA) has been widely used as a bone graft substitute. In this study, we investigated whether the addition of osteogenic protein-1 (OP-1) further enhanced the weak osteoinductive properties of hydroxyapatite when loaded with human mesenchymal stem cells (h-MSCs). Over a 14 day period, cell proliferation in both groups was assessed qualitatively using SEM and quantitatively using alamar blue assay. Cell differentiation was also evaluated by measurement of ALP activity, which was expressed against total DNA. HA/MSC loaded with OP-1 demonstrated a statistically significant increase ( p < 0.001) in cell proliferation at all time points in comparison to unloaded samples. ALP activity per DNA was also significantly enhanced ( p < 0.001) in loaded samples when compared to unloaded controls. SEM demonstrated increased cellular attachment and proliferation into HA pores at all time points in the loaded samples. Our study suggests that the osteoinductive potential of HA can be improved in vitro by the combined incorporation of MSCs and OP-1.