Long noncoding RNA POU6F2-AS1 regulates lung cancer aggressiveness through sponging miR-34c-5p to modulate KCNJ4 expression

It has been extensively reported that long noncoding RNAs (lncRNAs) were closely associated with multiple malignancies. The aim of our study was to investigate the effects and mechanism of lncRNA POU6F2-AS1 in lung adenocarcinoma (LADC).The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO...

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Published inGenetics and molecular biology Vol. 44; no. 2; p. e20200050
Main Authors Wu, Xiao-Yan, Xie, Yi, Zhou, Li-Yun, Zhao, Yuan-Yuan, Zhang, Jing, Zhang, Xiu-Feng, Guo, Shuai, Yu, Xue-Yan
Format Journal Article
LanguageEnglish
Published Brazil Sociedade Brasileira de Genética 01.01.2021
Subjects
BIOCHEMISTRY & MOLECULAR BIOLOGY
cell aggressiveness
GENETICS & HEREDITY
Human and Medical Genetics
KCNJ4
lung adenocarcinoma
miR-34c-5p
POU6F2-AS1
KCNJ4
POU6F2-AS1
cell aggressiveness
miR-34c-5p
lung adenocarcinoma
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Summary:It has been extensively reported that long noncoding RNAs (lncRNAs) were closely associated with multiple malignancies. The aim of our study was to investigate the effects and mechanism of lncRNA POU6F2-AS1 in lung adenocarcinoma (LADC).The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets provided us the information of LADC clinical samples. High-regulation of POU6F2-AS1 was presented in LADC tissues compared with adjacent normal tissues, which was correlated with poor outcome of LADC patients. Functional experiments in Calu-3 and NCI-H460 cells showed that POU6F2-AS1 significantly promoted LADC cell proliferation, colony formation, invasion and migration. Moreover, through online prediction, luciferase reporter assay and Pearson's correlation analysis, we found that POU6F2-AS1 may act as a competing endogenous RNA (ceRNA) of miR-34c-5p and facilitated the expression of potassium voltage-gated channel subfamily J member 4 (KCNJ4). The promoting effect of cell aggressiveness induced by POU6F2-AS1 was enhanced by KCNJ4, whilst was abrogated due to the overexpression of miR-34c-5p. Collectively, POU6F2-AS1 might function as a ceRNA through sponging miR-34c-5p to high-regulate KCNJ4 in LADC, which indicates that POU6F2-AS1 might be a promising therapeutic target with significant prognostic value for LADC treatment.
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Conflict of interests: The authors declare that there is no conflict of interest that could be perceived as prejudicial to the impartiality of the reported research.
Associate editor: Maria Angélica Cortez
Authors Contributions: XYW and XYY initiated and designed the study; XYW, YX, LZ, YYZ, JZ, and SG performed experiments; YX, XYW and XFZ analyzed data. XYW draft this article and XYY revised the manuscript. They all read and approved the final manuscript.
ISSN:1415-4757
1678-4685
1678-4685
DOI:10.1590/1678-4685-GMB-2020-0050