IMMUNOHISTOCHEMICAL LOCALIZATION OF DIPEPTIDYL AMINOPEPTIDASE (DAP) IV IN THE RAT ENDOCRINE ORGANS
Immunohistochemical localization of dipeptidyl aminopeptidase (DAP) IV in the rat endocrine organs was studied using the unlabeled antibody method. Fresh frozen, chloroform-acetone treated sections of the endocrine organs (pineal, pituitary, thyroid, parathyroid, adrenal gland and pancreas) were inv...
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| Published in | ACTA HISTOCHEMICA ET CYTOCHEMICA Vol. 14; no. 6; pp. 581 - 587 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
Sendai
JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
01.01.1981
Japan Science and Technology Agency |
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| Abstract | Immunohistochemical localization of dipeptidyl aminopeptidase (DAP) IV in the rat endocrine organs was studied using the unlabeled antibody method. Fresh frozen, chloroform-acetone treated sections of the endocrine organs (pineal, pituitary, thyroid, parathyroid, adrenal gland and pancreas) were investigated for the present study. A fairly intense immunoreaction of DAP IV was detected in the capillary endothelium of the thyroid gland and adrenal cortex. The capillary endothelium of the pineal, pituitary, parathyroid gland and pancreatic islets showed faint immunoreaction. No immunoreaction could be seen in the parenchymal cells of these endocrine organs except for the follicular epithelial cells of the thyroid gland. These results indicated that DAP IV in the endocrine organs was restricted to the capillary endothelium, and showed organ-dependent differences. Especially, it seemed that DAP IV in the capillary endothelium might be of importance in the thyroid and adrenal glands. |
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| AbstractList | Immunohistochemical localization of dipeptidyl aminopeptidase (DAP) IV in the rat endocrine organs was studied using the unlabeled antibody method. Fresh frozen, chloroform-acetone treated sections of the endocrine organs (pineal, pituitary, thyroid, parathyroid, adrenal gland and pancreas) were investigated for the present study. A fairly intense immunoreaction of DAP IV was detected in the capillary endothelium of the thyroid gland and adrenal cortex. The capillary endothelium of the pineal, pituitary, parathyroid gland and pancreatic islets showed faint immunoreaction. No immunoreaction could be seen in the parenchymal cells of these endocrine organs except for the follicular epithelial cells of the thyroid gland. These results indicated that DAP IV in the endocrine organs was restricted to the capillary endothelium, and showed organ-dependent differences. Especially, it seemed that DAP IV in the capillary endothelium might be of importance in the thyroid and adrenal glands. |
| Author | HARADA, MINORU SUZUKI, KAZUO FUKASAWA, KATSUHIKO SAHARA, NORIYUKI |
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| Copyright | the Japan Society of Histochemistry and Cytochemistry Copyright Japan Science and Technology Agency 1981 |
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| References | 17. Sahara, N., Fukasawa, K. M., Fukasawa, K., Araki, N. and Suzuki, K.: Immunohistochemical localization of dipeptidyl aminopeptidase (DAP) IV in the rat submandibular gland during postnatal development. Histochemistry 72; 229, 1981. 10. Kato, T., Nagatsu, T., Fukasawa, K., Harada, M., Nagatsu, I. and Sakakibraa, S.: Successive cleavage of N-terminal Arg1-Pro2 and Lys3-Pro4 from substance P but no release of Arg1-Pro2 from bradykinin, by X-Pro dipeptidyl-aminopeptidase. Biochim. Biophys. Acta 525; 417, 1978. 12. Lojda, Z.: Studies on dipeptidyl (amino) peptidase IV (glycylproline naphthylamidase). II. Blood vessels. Histochemistry 59; 153, 1979. 15. Oya, H., Nagatsu, I. and Nagatsu, T.: Purification and properties of glycylprolyl, β-naphthylamidase in human submaxillary gland. Biochim. Biophys. Acta 258; 591, 1972. 1. Fukasawa, K. M., Fukasawa, K. and Harada, M.: Dipeptidyl aminopeptidase IV, a glycoprotein from pig kidney. Biochim. Biophys. Acta 535; 161, 1978. 2. Fukasawa, K. M., Fukasawa, K., Hiraoka, B. Y. and Harada, M.: Comparison of dipeptidyl peptidase IV prepared from pig liver and kidney. Biochim. Biophys. Acta 657; 179, 1981. 14. McDonald, J. K., Callahan, P. X., Ellis, S. and Smith, R. E.: Polypeptide degradation by dipeptidylaminopeptidase I (cathepsin C) and related peptidase. In Tissue Proteinases, ed. by A. J. Barrett & J. T. Dingle, North-Holland Publishing Co, Amsterdam, 1971, p. 69. 19. Svensson, B., Danielsen, M., Staun, M., Jeppesen, L., Norén, O. and Sjöström, H.: An amphiphilic form of dipeptidyl peptidase IV from pig small-intestinal brush border membrane, purification by immunoadsorbent chromatography and some properties. Eur. J. Biochem. 90; 489, 1978. 16. Rhodin, J. A. G.: The ultrastructure of the adrenal cortex of the rat under normal and experimental conditions. J. Ultrastruct. Res. 34; 23, 1971. 3. Fukasawa, K. M., Fukasawa, K., Sahara, N., Harada, M., Kondo, Y. and Nagatsu, I.: Immunohistochemical localization of dipeptidyl aminopeptidase IV in rat kidney, liver and salivary glands. J. Histochem. Cytochem. 29; 337, 1981. 8. Hopsu-Havu, V. K. and Ekfors, T. O.: Distribution of a dipeptide naphthylamidase in rat tissues and its localization by using diazo coupling and labeled antibody techniques. Histochemie 17; 30, 1969. 6. Hopsu-Havu, V. K. and Sarimo, S. R.: Purification and characterization of an aminopeptidase hydrolyzing glycyl-proline-naphthylamide. Hoppe-Seyler's Z. Physiol. Chem. 348; 1540, 1967. 18. Sternberger, L. A.: The unlabeled antibody enzyme method. In Immunocytochemistry, ed. by L. A., Sternberger, Prentice-Hall, New Jersey, 1974, p. 129. 11. Lojda, Z.: Studies on glycyl-proline naphthylamidase. I. Lymphocytes. Histochemistry 54; 299, 1977. 9. Kato, T., Nagatsu, T., Kimura, T. and Sakakibara, S.: Fluorescence assay of X-prolyl dipeptidyl-aminopeptidase activity with a new fluorogenic substrate. Biochem. Med. 19; 351, 1978. 7. Hopsu-Havu, V. K., Rintola, P. and Glenner, G. G.: A hog kidney aminopeptidase liberating N-terminal dipeptides. Partial purification and characteristics. Acta Chem. Scand. 22; 299, 1968. 20. Wurtman, R. J. and Axelrod, J.: Control of enzymatic synthesis of adrenaline in the adrenal medulla by adrenal cortical steroids. J. Biol. Chem. 241; 2301, 1966. 4. Gossrau, R.: Peptidasen II. Zur Lokalisation der Dipeptidylpeptidase IV (DPP IV). Histochemische und biochemische Untersuchung. Histochemistry 60; 231, 1979. 13. Lowry, O. H., Rosenbrough, N. J., Farr, A. L. and Randall, R. J.: Protein measurement with the folin phenol reagent. J. Biol. Chem. 193; 265, 1951. 21. Yoshimoto, T. and Walter, R.: Post-proline dipeptidyl aminopeptidase (dipeptidyl aminopeptidase IV) from lamb kidney, purification and some enzymatic properties. Biochim. Biophys. Acta 485; 391, 1977. 5. Hopsu-Havu, V. K. and Glenner, G. G.: A new dipeptide naphthylamidase hydrolyzing glycyl-prolyl-β-naphthylamide. Histochemie 7; 197, 1966. |
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| Title | IMMUNOHISTOCHEMICAL LOCALIZATION OF DIPEPTIDYL AMINOPEPTIDASE (DAP) IV IN THE RAT ENDOCRINE ORGANS |
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