Suppression of NS3 and MP is important for the stable inheritance of RNAi-mediated Rice Stripe Virus (RSV) resistance obtained by targeting the fully complementary RSV-CP gene

• Published in: Molecules and cells Vol. 33; no. 1; pp. 43 - 51
• Main Authors: Park, H.M., National Institute of Crop Science, RDA, Suwon, Republic of Korea, Choi, M.S., National Institute of Crop Science, RDA, Suwon, Republic of Korea, Kwak, D.Y., National Institute of Crop Science, RDA, Milyang, Republic of Korea, Lee, B.C., National Institute of Crop Science, RDA, Suwon, Republic of Korea, Lee, J.H., National Institute of Crop Science, RDA, Milyang, Republic of Korea, Kim, M.K., National Institute of Crop Science, RDA, Suwon, Republic of Korea, Kim, Y.G., National Institute of Crop Science, RDA, Suwon, Republic of Korea, Shin, D.B., National Institute of Crop Science, RDA, Suwon, Republic of Korea, Park, S.K., Kyungpook National University, Daegu, Republic of Korea, Kim, Y.H., National Institute of Crop Science, RDA, Suwon, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Springer Korean Society for Molecular and Cellular Biology 01.01.2012; Korea Society for Molecular and Cellular Biology; 한국분자세포생물학회
• Subjects: Biochemistry; Biomedical and Life Sciences; Biomedicine; Biotechnology; Capsid Proteins - genetics; Cell Biology; Gene Silencing; Genes, Viral; Genetic Predisposition to Disease; Life Sciences; movement protein; Oryza - genetics; ORYZA SATIVA; Plant Diseases - genetics; Plant Diseases - prevention & control; Plant Diseases - virology; Plants, Genetically Modified; rice stripe virus; RNA Interference; RNA, Plant - genetics; RNA, Small Interfering - genetics; RNAi; RSV coat protein; silencing suppressor; small brown planthopper; Tenuivirus - genetics; Tenuivirus - metabolism; Viral Nonstructural Proteins - genetics; 생물학; RSV coat protein; RNAi; rice stripe virus (RSV); small brown planthopper (SBPH); movement protein (MP); L; silencing suppressor (NS3)
• ISSN: 1016-8478; 0219-1032
• DOI: 10.1007/s10059-012-2185-5

Rice stripe virus (RSV) is a viral disease that seriously impacts rice production in East Asia, most notably in Korea, China, and Japan. Highly RSV-resistant transgenic japonica rice plants were generated using a dsRNAi construct designed to silence the entire sequence region of the RSV-CP gene. Transgenic rice plants were inoculated with a population of viruliferous insects, small brown planthoppers (SBPH), and their resistance was evaluated using ELISA and an infection rate assay. A correlation between the expression of the RSV-CP homologous small RNAs and the RSV resistance of the transgenic rice lines was discovered. These plants were also analyzed by comparing the expression pattern of invading viral genes, small RNA production and the stable transmission of the RSV resistance trait to the T3 generation. Furthermore, the agronomic trait was stably transmitted to the T4 generation of transgenic plants.