Localization of Eosinophil Granule Major Basic Protein in Chronic Urticaria

The role of the eosinophil in the pathogenesis of cutaneous diseases is not known. The eosinophil granule major basic protein (MBP), constituting the core and accounting for greater than 50% of the eosinophil granule, is toxic to helminths and mammalian cells. To determine whether eosinophil degranu...

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Published inJournal of investigative dermatology Vol. 81; no. 1; pp. 39 - 43
Main Authors Peters, Margot S., Schroeter, Arnold L., Kephart, Gail M., Gleich, Gerald J.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.07.1983
Subjects
Adolescent
Adult
Aged
Blood Proteins - metabolism
Child
Child, Preschool
Chronic Disease
Cytoplasmic Granules - metabolism
Eosinophil Granule Proteins
Eosinophils - metabolism
Eosinophils - ultrastructure
Female
Fluorescent Antibody Technique
Histocytochemistry
Humans
Male
Middle Aged
Ribonucleases
Skin - metabolism
Urticaria - metabolism
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Summary:The role of the eosinophil in the pathogenesis of cutaneous diseases is not known. The eosinophil granule major basic protein (MBP), constituting the core and accounting for greater than 50% of the eosinophil granule, is toxic to helminths and mammalian cells. To determine whether eosinophil degranulation occurs in lesions of chronic urticaria, we performed an indirect immuno-fluorescence assay on sections of formalin-fixed, paraffin-embedded tissue, utilizing affinity chromatography-purified antibody to MBP. Twelve of 28 biopsies showed evidence of degranulation as judged by the deposition of MBP outside the eosinophil. The positive staining was of 3 types: (1) small blood vessel walls (5 patients), (2) dispersion of granular material (9 patients), and (3) focal or diffuse immunofluorescence of connective tissue fibers (11 patients). These results suggest a possible role for the cytotoxic molecule MBP in the evolution of lesions of chronic urticaria.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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ISSN:0022-202X
1523-1747
DOI:10.1111/1523-1747.ep12538380