The Dual-Targeted Peptide Conjugated Probe for Depicting Residual Nasopharyngeal Carcinoma and Guiding Surgery

• Published in: Biosensors (Basel) Vol. 12; no. 9; p. 729
• Main Authors: Huang, Wenhui, He, Zicong, Cai, Xuekang, Zhang, Jingming, Li, Wei, Wang, Kun, Zhang, Shuixing
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.09.2022; MDPI
• Subjects: Acoustooptical devices; Antibodies; Biomarkers; Biomedical materials; Care and treatment; Coexistence; Computer-assisted surgery; Diagnosis; Epidermal growth factor; ErbB-2 protein; Experiments; Flow cytometry; Fluorescence; fluorescence molecular imaging; HER2; Imaging techniques; imaging-guided surgery; In vivo methods and tests; Laboratory animals; Medical research; Methods; Nasopharyngeal cancer; Nasopharyngeal carcinoma; Optical properties; optoacoustic imaging; Peptides; Radiation therapy; Reagents; SR-BI; Surgery; Throat cancer; Tumors; China; Beijing China; United States > US; Germany
• ISSN: 2079-6374; 2079-6374
• DOI: 10.3390/bios12090729

Detecting residual nasopharyngeal carcinoma (rNPC) can be difficult because of the coexistence of occult tumours and post-chemoradiation changes, which poses a challenge for both radiologists and surgeons using current imaging methods. Currently, molecular imaging that precisely targets and visualises particular biomarkers in tumours may exceed the specificity and sensitivity of traditional imaging techniques, providing the potential to distinguish tumours from non-neoplastic lesions. Here, we synthesised a HER2/SR-BI-targeted tracer to efficiently position NPC and guide surgery in living mice. This bispecific tracer contained the following two parts: IRDye 800 CW, as an imaging reagent for both optical and optoacoustic imaging, and a fusion peptide (FY-35), as the targeting reagent. Both in vitro and in vivo tests demonstrated that the tracer had higher accumulation and longer retention (up to 48 h) in tumours than a single-targeted probe, and realised sensitive detection of tumours with a minimum size of 3.9 mm. By visualising the vascular network via a customised handheld optoacoustic scan, our intraoperative fluorescence molecular imaging system provides accurate guidance for intraoperative tumour resection. Integrating the advantages of both optical and optoacoustic scanning in an intraoperative image-guided system, this method holds promise for depicting rNPC and guiding salvage surgery.