Immobilization of bovine serum albumin onto porous polyethylene membranes using strongly attached polydopamine as a spacer

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 86; no. 1; pp. 111 - 118
• Main Authors: Zhu, Li-Ping, Jiang, Jin-Hong, Zhu, Bao-Ku, Xu, You-Yi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2011
• Subjects: adhesion; Animals; aqueous solutions; Blood; blood platelets; Bovine serum albumin; Cattle; cell adhesion; cell culture; cell viability; Coating; coatings; colloids; contact angle; dipping; Dopamine; Dopamine - chemistry; Fourier transform infrared spectroscopy; hydrophilicity; Immobilization; Membranes; Membranes, Artificial; Models, Theoretical; PDA; Polyethylene; Polyethylene - chemistry; Polyethylenes; Polymers - chemistry; Porosity; Porous membranes; Serum albumin; Serum Albumin, Bovine - chemistry; Surface modification; Surface Properties; X-ray photoelectron spectroscopy; Surface modification; Bovine serum albumin; Polyethylene; Dopamine; Porous membranes
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2011.03.027

. [Display omitted] ► Porous PE membrane was successfully surface-modified via dopamine self-polymerization and firm attachment to membrane surface followed by BSA binding in mild aqueous environments. ► The hydrophilicity of PE membrane was significantly improved by the polydopamine coating and BSA immobilization. ► The BSA-immobilized PE membrane had better blood compatibility than the unmodified PE and the PE/pDA composite membranes. ► Compared to BSA surface, polydopamine surface is more favorable for cell adhesion, growth, and proliferation. Based on the self-polymerization and strong adhesion characteristics of dopamine in aqueous solution, a novel and convenient approach was developed to immobilize protein onto porous polyethylene (PE) membranes. A thin polydopamine (pDA) layer was formed and tightly coated onto PE membrane by dipping simply the membrane into dopamine aqueous solution for a period of time. Subsequently, bovine serum albumin (BSA) was bound onto the obtained PE/pDA composite membranes via the coupling between BSA and the reactive polydopamine layer. The firm immobilization of polydopamine layer and BSA was verified by attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS). The results of water contact angle measurement showed that the hydrophilicity of PE membrane was significantly improved after coating polydopamine and binding BSA. The experiments of blood platelet adhesion indicated that BSA-immobilized PE membrane had better blood compatibility than the unmodified PE and the PE/pDA composite membranes. The investigations on hepatocyte cultures and cell viability revealed that the polydopamine coating endowed PE membrane with significantly improved cell compatibility. Compared to BSA surface, polydopamine surface is more favorable for cell adhesion, growth, and proliferation.