Increased uncoupling proteins and decreased efficiency in palmitate-perfused hyperthyroid rat heart
1 Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom; and 2 Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, Rockville, Maryland 20852 The physiological role of mitochondrial uncoupling proteins (UCPs) in heart an...
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Published in | American journal of physiology. Heart and circulatory physiology Vol. 280; no. 3; pp. H977 - H983 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.03.2001
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Subjects | |
Online Access | Get full text |
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Summary: | 1 Department of Biochemistry, University of Oxford, Oxford
OX1 3QU, United Kingdom; and 2 Laboratory of Membrane
Biochemistry and Biophysics, National Institute on Alcohol Abuse and
Alcoholism, Rockville, Maryland 20852
The physiological
role of mitochondrial uncoupling proteins (UCPs) in heart and skeletal
muscle is unknown, as is whether mitochondrial uncoupling of oxidative
phosphorylation by fatty acids occurs in vivo. In this
study, we found that UCP2 and UCP3 protein content, determined using
Western blotting, was increased by 32 and 48%, respectively, in
hyperthyroid rat heart mitochondria. Oligomycin-insensitive respiration
rate, a measure of mitochondrial uncoupling, was increased in all
mitochondria in the presence of palmitate: 36% in controls and 71 and
100% with 0.8 and 0.9 mM palmitate, respectively, in hyperthyroid rat
heart mitochondria. In the isolated working heart, 0.4 mM palmitate
significantly lowered cardiac output by 36% and cardiac efficiency by
38% in the hyperthyroid rat heart. Thus increased mitochondrial UCPs in the hyperthyroid rat heart were associated with increased uncoupling and decreased myocardial efficiency in the presence of palmitate. In
conclusion, a physiological effect of UCPs on fatty acid oxidation has
been found in heart at the mitochondrial and whole organ level.
isolated mitochondria; cardiac efficiency |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0363-6135 1522-1539 |
DOI: | 10.1152/ajpheart.2001.280.3.h977 |