Large Particle Fluorescence-Activated Cell Sorting Enables High-Quality Single-Cell RNA Sequencing and Functional Analysis of Adult Cardiomyocytes

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Bibliographic Details
Published inCirculation research Vol. 125; no. 5; pp. 567 - 569
Main Authors Kannan, Suraj, Miyamoto, Matthew, Lin, Brian Leei, Zhu, Renjun, Murphy, Sean, Kass, David A, Andersen, Peter, Kwon, Chulan
Format Journal Article
LanguageEnglish
Published United States American Heart Association, Inc 16.08.2019
Subjects
Age Factors
Animals
Flow Cytometry - methods
Mice
Myocytes, Cardiac - chemistry
Myocytes, Cardiac - physiology
Sequence Analysis, RNA - methods
Single-Cell Analysis - methods
perfusion
sarcomeres
fluorescence
microfluidics
staining
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Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Correspondence-1
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AUTHOR CONTRIBUTIONS
S.K., M.M., and B.L. performed experiments. R.Z., D.K., P.A., S.M., and C.K. provided intellectual contribution. The manuscript was initially written by S.K. with subsequent inputs from all authors.
ISSN:0009-7330
1524-4571
1524-4571
DOI:10.1161/CIRCRESAHA.119.315493