Fragmentation of cellulose by the major Thermomonospora fusca cellulases, Trichoderma reesei CBHI, and their mixtures

In this study, the fragmentation activities of Thermomonospora fusca cellulases E2, E3, E5, Trichoderma reesei CBHI, and their mixtures were measured to study synergism in fragmentation. Fragmentation studies revealed that only two pure cellulases, T. fusca E2 and E5 had significant fragmentation ac...

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Published inBiotechnology and bioengineering Vol. 40; no. 9; p. 1019
Main Authors Walker, L.P. (Cornell University, Ithaca, NY), Wilson, D.B, Irwin, D.C, McQuire, C, Price, M
Format Journal Article
LanguageEnglish
Published United States 01.11.1992
Subjects
ACTINOMYCETALES
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
CELLULASE
CELLULOSE
CELULASA
CELULOSA
HIDROLISIS
HYDROLYSE
MICROORGANISME THERMOPHILE
MICROORGANISMOS TERMOFILOS
SINERGISMO
SYNERGIE
TRICHODERMA
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Summary:In this study, the fragmentation activities of Thermomonospora fusca cellulases E2, E3, E5, Trichoderma reesei CBHI, and their mixtures were measured to study synergism in fragmentation. Fragmentation studies revealed that only two pure cellulases, T. fusca E2 and E5 had significant fragmentation activity. T. fusca E3 shows strong synergism both in the production of reducing sugars and in fragmentation with both T. fusca endoglucanases and with T. reesei CBHI. Most mixtures containing CBHI produced higher rates of fragmentation than comparable mixtures containing E3. The highest rate and extent of reducing sugar formation and the highest fragmentation activity were obtained with a mixture of E2, E3, and CBHI
Bibliography:9406592
F60
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260400905