Cloning and sequencing of the ovine gamma-interferon gene [cytokine; immune system regulation]

Gamma- interferon was selected as an example of important cytokines in the regulation of the immune system of the sheep. By the use of the polymerase chain reaction (PCR), using primers based on the bovine gamma- interferon sequence, the ovine gene was amplified from crude messenger RNA extracted fr...

Full description

Saved in:
Bibliographic Details
Published inAustralian veterinary journal Vol. 68; no. 3; p. 82
Main Authors Radford, A.J, Hodgson, A.L.M, Rothel, J.S, Wood, P.R. (Commonwealth Scientific and Industrial Research Organisation, Parkville (Australia). Div. of Animal Health)
Format Journal Article
LanguageEnglish
Published England 01.03.1991
Subjects
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
DNA - chemistry
GENE
GENES
HORMONAS
HORMONE
INTERFERON
Interferon-gamma - chemistry
Interferon-gamma - genetics
INTERFERONAS
Molecular Sequence Data
OVIN
OVINOS
Polymerase Chain Reaction
PROTEINAS
PROTEINE
REPONSE IMMUNITAIRE
RESPUESTA INMUNOLOGICA
SECUENCIA NUCLEICA
Sequence Homology, Nucleic Acid
SEQUENCE NUCLEIQUE
Sheep - genetics
Sheep - immunology
Online AccessGet more information

Cover

More Information
Summary:Gamma- interferon was selected as an example of important cytokines in the regulation of the immune system of the sheep. By the use of the polymerase chain reaction (PCR), using primers based on the bovine gamma- interferon sequence, the ovine gene was amplified from crude messenger RNA extracted from lymphocytes. It was found that ovine gamma- interferon is 93 percent identical with bovine in amino acid sequence. This result indicates that the PCR method will be a rapid and efficient means for cloning other ovine cytokine genes.
Bibliography:9102057
L73
L10
ISSN:0005-0423
1751-0813
DOI:10.1111/j.1751-0813.1991.tb00755.x