Lobelia trigona Roxb-based nanomedicine with enhanced biological applications: in vitro and in vivo approach

• Published in: IET nanobiotechnology Vol. 14; no. 8; pp. 739 - 748
• Main Authors: Samdavid Thanapaul, Rex Jeya Rajkumar, Manikandan, Sreeraj K, Govindaraj, Tamil Selvan, Selvakumar, Paulraj Mosae, Enoch, Israel V.M.V, Muthukumar Nadar, Meenakshi Subbaraman Adyanpuram
• Format: Journal Article
• Language: English
• Published: United States The Institution of Engineering and Technology 01.10.2020
• Subjects: A549 human cancer cell lines; Animals; Anti-Bacterial Agents - administration & dosage; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; antibacterial activity; antibacterial properties; anticancer activity; antifungal properties; antiinflammatory effects; Antineoplastic Agents - administration & dosage; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Antioxidants - administration & dosage; Antioxidants - chemistry; Antioxidants - pharmacology; antiproliferative effects; Bacteria - drug effects; biochemistry; biological applications; biomedical materials; burn wound‐healing activity; Burns - drug therapy; Burns - pathology; cancer; cellular biophysics; comet assay; DNA Damage; dynamic light scattering; energy dispersive X‐ray analysis; enzymes; excision; formulated LTAgNPs ointment; Fourier transform infrared spectra; Fourier transform infrared spectroscopy; free radical scavenging; Fungi - drug effects; genotoxicity effects; HeLa human cancer cell lines; in vitro approach; In Vitro Techniques; in vivo approach; lactate dehydrogenase release assay; light scattering; Lobelia - chemistry; Lobelia trigona Roxb‐based nanomedicine; Lobelia trigona Roxb‐mediated silver nanoparticles; Male; MCF‐7 human cancer cell lines; MDA‐MB‐231 human cancer cell lines; Metal Nanoparticles - administration & dosage; Metal Nanoparticles - chemistry; microorganisms; molecular biophysics; nanofabrication; Nanomedicine; nanoparticles; Neoplasms - drug therapy; Neoplasms - pathology; nitric oxide inhibitory assay; optical properties; pathogens; Plant Extracts - pharmacology; power assay; Rats; Rats, Wistar; Research Article; scanning electron microscopy; scavenging effects; silver; Silver - chemistry; strains; structural properties; toxicology; Tumor Cells, Cultured; ultraviolet spectra; ultraviolet‐visible spectroscopy; visible spectra; Wound Healing - drug effects; wounds; antibacterial activity; cellular biophysics; burn wound-healing activity; genotoxicity effects; visible spectra; nanoparticles; Lobelia trigona Roxb-mediated silver nanoparticles; HeLa human cancer cell lines; scanning electron microscopy; excision; dynamic light scattering; wounds; power assay; MCF-7 human cancer cell lines; structural properties; optical properties; formulated LTAgNPs ointment; biomedical materials; silver; Fourier transform infrared spectroscopy; anticancer activity; MDA-MB-231 human cancer cell lines; antifungal properties; Fourier transform infrared spectra; Lobelia trigona Roxb-based nanomedicine; Ag; nanofabrication; scavenging effects; enzymes; antiproliferative effects; light scattering; strains; ultraviolet-visible spectroscopy; free radical scavenging; pathogens; nitric oxide inhibitory assay; antiinflammatory effects; toxicology; A549 human cancer cell lines; in vitro approach; nanomedicine; antibacterial properties; biological applications; energy dispersive X-ray analysis; in vivo approach; ultraviolet spectra; comet assay; microorganisms; biochemistry; lactate dehydrogenase release assay; cancer; molecular biophysics
• ISSN: 1751-8741; 1751-875X; 1751-875X
• DOI: 10.1049/iet-nbt.2020.0148

This is the first study to report the green synthesis of Lobelia trigona Roxb-mediated silver nanoparticles (LTAgNPs). The optical and structural properties of the synthesised LTAgNPs were analysed using ultraviolet–visible spectroscopy, scanning electron microscopy, Fourier transform infrared, dynamic light scattering and energy dispersive X-ray. LTAgNps were evaluated for their anti-bacterial and anti-fungal properties against 18 pathogens and exhibited significant inhibition against all the strains tested. LTAgNPs had potential scavenging effects on the DPPH, •OH, O2•− free radical scavenging assays and reducing power assay. LTAgNps possess strong anti-cancer activity against five human cancer cell lines (A549, MCF-7, MDA-MB-231, HeLa and KB) in a dose-dependent manner. The antiproliferative, anti-inflammatory and genotoxicity effects of LTAgNPs were further confirmed by the lactate dehydrogenase release assay, nitric oxide inhibitory assay and comet assay. Furthermore, the incision, excision and burn wound-healing activity of formulated LTAgNPs ointment was assessed in rats. All the wounds had significant healing in groups treated with LTAgNPs ointment compared to the groups treated with the commonly prescribed ointment (SilverexTM). This study shows and suggests that the previously unreported LTAgNPs could be used as a nanomedicine with significant biological applications.